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Comparison Study of Oral Iron Preparations Using a Human Intestinal Model

Iron deficiency and related iron deficiency anaemia (IDA) are the most prevalent nutritional disorders worldwide. The standard treatment involves supplementation with solid or liquid iron supplement preparations, usually based on a ferrous salt such as ferrous sulphate, ferrous fumarate, or ferrous...

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Autores principales: Zariwala, Mohammed Gulrez, Somavarapu, Satyanarayana, Farnaud, Sebastien, Renshaw, Derek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Österreichische Apotheker-Verlagsgesellschaft 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867244/
https://www.ncbi.nlm.nih.gov/pubmed/24482777
http://dx.doi.org/10.3797/scipharm.1304-03
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author Zariwala, Mohammed Gulrez
Somavarapu, Satyanarayana
Farnaud, Sebastien
Renshaw, Derek
author_facet Zariwala, Mohammed Gulrez
Somavarapu, Satyanarayana
Farnaud, Sebastien
Renshaw, Derek
author_sort Zariwala, Mohammed Gulrez
collection PubMed
description Iron deficiency and related iron deficiency anaemia (IDA) are the most prevalent nutritional disorders worldwide. The standard treatment involves supplementation with solid or liquid iron supplement preparations, usually based on a ferrous salt such as ferrous sulphate, ferrous fumarate, or ferrous gluconate. In the present study, we compared iron uptake and absorption from various solid and liquid iron supplement preparations currently available in the United Kingdom using the well-characterised human epithelial adenocarcinoma cell line Caco-2. Intracellular ferritin protein formation by the Caco-2 cell was considered an indicator of cellular iron uptake and absorption. We investigated the effects of formulation ingredients at a defined pH on iron uptake and absorption, and designed a novel two-stage dissolution-absorption protocol that mimicked physiological conditions. Our experiments revealed wide variations in the rate of dissolution between the various solid iron preparations. Conventional-release ferrous iron tablets dissolved rapidly (48 ± 4 mins to 64 ± 4 mins), whereas modified-released tablets and capsules took significantly longer to undergo complete dissolution (274 ± 8 to 256 ± 8 mins). Among the solid iron preparations, ferrous sulphate conventional-release tablets demonstrated the highest iron absorption, whereas modified-release ferrous preparations demonstrated uniformly low iron absorption, as compared to the control (P < 0.05). Taken together, our results demonstrate that there are wide-ranging variations in dissolution times and iron uptake from oral iron preparations, with the physical characteristics of the preparation as well as the form of iron playing a key role.
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spelling pubmed-38672442014-01-30 Comparison Study of Oral Iron Preparations Using a Human Intestinal Model Zariwala, Mohammed Gulrez Somavarapu, Satyanarayana Farnaud, Sebastien Renshaw, Derek Sci Pharm Research Article Iron deficiency and related iron deficiency anaemia (IDA) are the most prevalent nutritional disorders worldwide. The standard treatment involves supplementation with solid or liquid iron supplement preparations, usually based on a ferrous salt such as ferrous sulphate, ferrous fumarate, or ferrous gluconate. In the present study, we compared iron uptake and absorption from various solid and liquid iron supplement preparations currently available in the United Kingdom using the well-characterised human epithelial adenocarcinoma cell line Caco-2. Intracellular ferritin protein formation by the Caco-2 cell was considered an indicator of cellular iron uptake and absorption. We investigated the effects of formulation ingredients at a defined pH on iron uptake and absorption, and designed a novel two-stage dissolution-absorption protocol that mimicked physiological conditions. Our experiments revealed wide variations in the rate of dissolution between the various solid iron preparations. Conventional-release ferrous iron tablets dissolved rapidly (48 ± 4 mins to 64 ± 4 mins), whereas modified-released tablets and capsules took significantly longer to undergo complete dissolution (274 ± 8 to 256 ± 8 mins). Among the solid iron preparations, ferrous sulphate conventional-release tablets demonstrated the highest iron absorption, whereas modified-release ferrous preparations demonstrated uniformly low iron absorption, as compared to the control (P < 0.05). Taken together, our results demonstrate that there are wide-ranging variations in dissolution times and iron uptake from oral iron preparations, with the physical characteristics of the preparation as well as the form of iron playing a key role. Österreichische Apotheker-Verlagsgesellschaft 2013 2013-06-21 /pmc/articles/PMC3867244/ /pubmed/24482777 http://dx.doi.org/10.3797/scipharm.1304-03 Text en © Zariwala et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zariwala, Mohammed Gulrez
Somavarapu, Satyanarayana
Farnaud, Sebastien
Renshaw, Derek
Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title_full Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title_fullStr Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title_full_unstemmed Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title_short Comparison Study of Oral Iron Preparations Using a Human Intestinal Model
title_sort comparison study of oral iron preparations using a human intestinal model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867244/
https://www.ncbi.nlm.nih.gov/pubmed/24482777
http://dx.doi.org/10.3797/scipharm.1304-03
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