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Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles...

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Autores principales: Vandooren, Jennifer, Berghmans, Nele, Dillen, Chris, Van Aelst, Ilse, Ronsse, Isabelle, Israel, Liron Limor, Rosenberger, Ina, Kreuter, Jörg, Lellouche, Jean-Paul, Michaeli, Shulamit, Locatelli, Erica, Franchini, Mauro Comes, Aiertza, Miren K, Sánchez-Abella, Laura, Loinaz, Iraida, Edwards, Dylan R, Shenkman, Louis, Opdenakker, Ghislain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867323/
https://www.ncbi.nlm.nih.gov/pubmed/24379662
http://dx.doi.org/10.2147/IJN.S51628
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author Vandooren, Jennifer
Berghmans, Nele
Dillen, Chris
Van Aelst, Ilse
Ronsse, Isabelle
Israel, Liron Limor
Rosenberger, Ina
Kreuter, Jörg
Lellouche, Jean-Paul
Michaeli, Shulamit
Locatelli, Erica
Franchini, Mauro Comes
Aiertza, Miren K
Sánchez-Abella, Laura
Loinaz, Iraida
Edwards, Dylan R
Shenkman, Louis
Opdenakker, Ghislain
author_facet Vandooren, Jennifer
Berghmans, Nele
Dillen, Chris
Van Aelst, Ilse
Ronsse, Isabelle
Israel, Liron Limor
Rosenberger, Ina
Kreuter, Jörg
Lellouche, Jean-Paul
Michaeli, Shulamit
Locatelli, Erica
Franchini, Mauro Comes
Aiertza, Miren K
Sánchez-Abella, Laura
Loinaz, Iraida
Edwards, Dylan R
Shenkman, Louis
Opdenakker, Ghislain
author_sort Vandooren, Jennifer
collection PubMed
description The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersensitivity reactions, and fibrosis. Here we show that artificial particles of specific sizes affect immune cell recruitment as tested in a dermal air pouch model in mice. In addition, we demonstrate that the composition of nanoparticles may influence immune cell recruitment in vivo. Aside from biophysical characterizations in terms of hydrodynamic diameter, zeta potential, concentration, and atomic concentration of metals, we show that – after first-line in vitro assays – characterization of cellular and molecular effects by dermal air pouch analysis is straightforward and should be included in the quality control of nanoparticles. We demonstrate this for innate immunological effects such as neutrophil recruitment and the production of immune-modulating matrix metalloproteases such as MMP-9; we propose the use of air pouch leukocytosis analysis as a future standard assay.
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spelling pubmed-38673232013-12-30 Intradermal air pouch leukocytosis as an in vivo test for nanoparticles Vandooren, Jennifer Berghmans, Nele Dillen, Chris Van Aelst, Ilse Ronsse, Isabelle Israel, Liron Limor Rosenberger, Ina Kreuter, Jörg Lellouche, Jean-Paul Michaeli, Shulamit Locatelli, Erica Franchini, Mauro Comes Aiertza, Miren K Sánchez-Abella, Laura Loinaz, Iraida Edwards, Dylan R Shenkman, Louis Opdenakker, Ghislain Int J Nanomedicine Original Research The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersensitivity reactions, and fibrosis. Here we show that artificial particles of specific sizes affect immune cell recruitment as tested in a dermal air pouch model in mice. In addition, we demonstrate that the composition of nanoparticles may influence immune cell recruitment in vivo. Aside from biophysical characterizations in terms of hydrodynamic diameter, zeta potential, concentration, and atomic concentration of metals, we show that – after first-line in vitro assays – characterization of cellular and molecular effects by dermal air pouch analysis is straightforward and should be included in the quality control of nanoparticles. We demonstrate this for innate immunological effects such as neutrophil recruitment and the production of immune-modulating matrix metalloproteases such as MMP-9; we propose the use of air pouch leukocytosis analysis as a future standard assay. Dove Medical Press 2013 2013-12-13 /pmc/articles/PMC3867323/ /pubmed/24379662 http://dx.doi.org/10.2147/IJN.S51628 Text en © 2013 Vandooren et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Vandooren, Jennifer
Berghmans, Nele
Dillen, Chris
Van Aelst, Ilse
Ronsse, Isabelle
Israel, Liron Limor
Rosenberger, Ina
Kreuter, Jörg
Lellouche, Jean-Paul
Michaeli, Shulamit
Locatelli, Erica
Franchini, Mauro Comes
Aiertza, Miren K
Sánchez-Abella, Laura
Loinaz, Iraida
Edwards, Dylan R
Shenkman, Louis
Opdenakker, Ghislain
Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title_full Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title_fullStr Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title_full_unstemmed Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title_short Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
title_sort intradermal air pouch leukocytosis as an in vivo test for nanoparticles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867323/
https://www.ncbi.nlm.nih.gov/pubmed/24379662
http://dx.doi.org/10.2147/IJN.S51628
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