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Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy
Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular comp...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867364/ https://www.ncbi.nlm.nih.gov/pubmed/24367525 http://dx.doi.org/10.1371/journal.pone.0082592 |
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author | Chiang, Wen Chih Lai, Chun Fu Su, Chi Ting Peng, Wei Hao Wu, Ching Fang Chang, Fan Chi Chen, Yi Ting Lin, Shuei Liong Chen, Yung Ming Wu, Kwan Dun Lu, Kuo Shyan Tsai, Tun Jun |
author_facet | Chiang, Wen Chih Lai, Chun Fu Su, Chi Ting Peng, Wei Hao Wu, Ching Fang Chang, Fan Chi Chen, Yi Ting Lin, Shuei Liong Chen, Yung Ming Wu, Kwan Dun Lu, Kuo Shyan Tsai, Tun Jun |
author_sort | Chiang, Wen Chih |
collection | PubMed |
description | Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor—tyrosine kinase with Ig and EGF homology domains-2—and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy. |
format | Online Article Text |
id | pubmed-3867364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38673642013-12-23 Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy Chiang, Wen Chih Lai, Chun Fu Su, Chi Ting Peng, Wei Hao Wu, Ching Fang Chang, Fan Chi Chen, Yi Ting Lin, Shuei Liong Chen, Yung Ming Wu, Kwan Dun Lu, Kuo Shyan Tsai, Tun Jun PLoS One Research Article Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor—tyrosine kinase with Ig and EGF homology domains-2—and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy. Public Library of Science 2013-12-18 /pmc/articles/PMC3867364/ /pubmed/24367525 http://dx.doi.org/10.1371/journal.pone.0082592 Text en © 2013 Chiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chiang, Wen Chih Lai, Chun Fu Su, Chi Ting Peng, Wei Hao Wu, Ching Fang Chang, Fan Chi Chen, Yi Ting Lin, Shuei Liong Chen, Yung Ming Wu, Kwan Dun Lu, Kuo Shyan Tsai, Tun Jun Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title | Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title_full | Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title_fullStr | Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title_full_unstemmed | Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title_short | Angiopoietins Modulate Endothelial Adaptation, Glomerular and Podocyte Hypertrophy after Uninephrectomy |
title_sort | angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867364/ https://www.ncbi.nlm.nih.gov/pubmed/24367525 http://dx.doi.org/10.1371/journal.pone.0082592 |
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