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Fluorescence Imaging-Based High-Throughput Screening of Fast- and Slow-Cycling LOV Proteins

Light-oxygen-voltage (LOV) domains function as blue light-inducible molecular switches. The photosensory LOV domains derived from plants and fungi have provided an indispensable tool for optogenetics. Here we develop a high-throughput screening system to efficiently improve switch-off kinetics of LO...

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Detalles Bibliográficos
Autores principales: Kawano, Fuun, Aono, Yuki, Suzuki, Hideyuki, Sato, Moritoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867380/
https://www.ncbi.nlm.nih.gov/pubmed/24367542
http://dx.doi.org/10.1371/journal.pone.0082693