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Fluorescence Imaging-Based High-Throughput Screening of Fast- and Slow-Cycling LOV Proteins
Light-oxygen-voltage (LOV) domains function as blue light-inducible molecular switches. The photosensory LOV domains derived from plants and fungi have provided an indispensable tool for optogenetics. Here we develop a high-throughput screening system to efficiently improve switch-off kinetics of LO...
Autores principales: | Kawano, Fuun, Aono, Yuki, Suzuki, Hideyuki, Sato, Moritoshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867380/ https://www.ncbi.nlm.nih.gov/pubmed/24367542 http://dx.doi.org/10.1371/journal.pone.0082693 |
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