Cargando…
Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness
We recently isolated and discovered new Bradyrhizobiaceae microbes from the cryopreserved culture broth of blood samples from 3 patients with poorly defined illnesses using modified SP4 media and culture conditions coupled with genomic sequencing. Using a similar protocol, we studied a previously cr...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867388/ https://www.ncbi.nlm.nih.gov/pubmed/24367538 http://dx.doi.org/10.1371/journal.pone.0082673 |
_version_ | 1782296294899843072 |
---|---|
author | Lo, Shyh-Ching Li, Bingjie Hung, Guo-Chiuan Lei, Haiyan Li, Tianwei Zhang, Jing Nagamine, Kenjiro Tsai, Shien Zucker, Mark J. Olesnicky, Ludmilla |
author_facet | Lo, Shyh-Ching Li, Bingjie Hung, Guo-Chiuan Lei, Haiyan Li, Tianwei Zhang, Jing Nagamine, Kenjiro Tsai, Shien Zucker, Mark J. Olesnicky, Ludmilla |
author_sort | Lo, Shyh-Ching |
collection | PubMed |
description | We recently isolated and discovered new Bradyrhizobiaceae microbes from the cryopreserved culture broth of blood samples from 3 patients with poorly defined illnesses using modified SP4 media and culture conditions coupled with genomic sequencing. Using a similar protocol, we studied a previously cryopreserved culture broth of blood sample from a patient who had succumbed to an acute onset of fulminant pulmonary illness. We report that two phases of microbial growth were observed in the re-initiated culture. Biochemical and genomic characterization revealed microbes isolated from the first phase of growth were new Afipia species of Bradyrhizobiaceae, tentatively named A. cberi with a ~ 5 MB chromosome that was different from those of all previously known Afipia microbes including the newly discovered A. septicemium. The microbes isolated from the second phase of growth were prominent sugar assimilators, novel Phyllobacteriaceae, phylogenetically most closely related to Mesorhizobium and tentatively named M. hominis with a ~ 5.5 MB chromosome. All A. cberi isolates carry a circular ~ 140 KB plasmid. Some M. hominis isolates possess a circular ~ 412 KB plasmid that can be lost in prolonged culture or passage. No antibiotics resistant genes could be identified in both of the A. cberi and M. hominis plasmids. Antibiotic susceptibility studies using broth culture systems revealed isolates of A. cberi could be sensitive to some antibiotics, but all isolates of M. hominis were resistant to essentially all tested antibiotics. However, the cell-free antibiotics susceptibility test results may not be applicable to clinical treatment against the microbes that are known to be capable of intracellular growth. It remains to be determined if the 2 previously unknown Rhizobiales were indeed pathogenic and played a role in the pulmonary disease process in this patient. Specific probes and methods will be developed to re-examine the diseased lungs from patient's autopsy. |
format | Online Article Text |
id | pubmed-3867388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38673882013-12-23 Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness Lo, Shyh-Ching Li, Bingjie Hung, Guo-Chiuan Lei, Haiyan Li, Tianwei Zhang, Jing Nagamine, Kenjiro Tsai, Shien Zucker, Mark J. Olesnicky, Ludmilla PLoS One Research Article We recently isolated and discovered new Bradyrhizobiaceae microbes from the cryopreserved culture broth of blood samples from 3 patients with poorly defined illnesses using modified SP4 media and culture conditions coupled with genomic sequencing. Using a similar protocol, we studied a previously cryopreserved culture broth of blood sample from a patient who had succumbed to an acute onset of fulminant pulmonary illness. We report that two phases of microbial growth were observed in the re-initiated culture. Biochemical and genomic characterization revealed microbes isolated from the first phase of growth were new Afipia species of Bradyrhizobiaceae, tentatively named A. cberi with a ~ 5 MB chromosome that was different from those of all previously known Afipia microbes including the newly discovered A. septicemium. The microbes isolated from the second phase of growth were prominent sugar assimilators, novel Phyllobacteriaceae, phylogenetically most closely related to Mesorhizobium and tentatively named M. hominis with a ~ 5.5 MB chromosome. All A. cberi isolates carry a circular ~ 140 KB plasmid. Some M. hominis isolates possess a circular ~ 412 KB plasmid that can be lost in prolonged culture or passage. No antibiotics resistant genes could be identified in both of the A. cberi and M. hominis plasmids. Antibiotic susceptibility studies using broth culture systems revealed isolates of A. cberi could be sensitive to some antibiotics, but all isolates of M. hominis were resistant to essentially all tested antibiotics. However, the cell-free antibiotics susceptibility test results may not be applicable to clinical treatment against the microbes that are known to be capable of intracellular growth. It remains to be determined if the 2 previously unknown Rhizobiales were indeed pathogenic and played a role in the pulmonary disease process in this patient. Specific probes and methods will be developed to re-examine the diseased lungs from patient's autopsy. Public Library of Science 2013-12-18 /pmc/articles/PMC3867388/ /pubmed/24367538 http://dx.doi.org/10.1371/journal.pone.0082673 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Lo, Shyh-Ching Li, Bingjie Hung, Guo-Chiuan Lei, Haiyan Li, Tianwei Zhang, Jing Nagamine, Kenjiro Tsai, Shien Zucker, Mark J. Olesnicky, Ludmilla Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title | Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title_full | Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title_fullStr | Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title_full_unstemmed | Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title_short | Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted with Fatal Pulmonary Illness |
title_sort | isolation and characterization of two novel bacteria afipia cberi and mesorhizobium hominis from blood of a patient afflicted with fatal pulmonary illness |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867388/ https://www.ncbi.nlm.nih.gov/pubmed/24367538 http://dx.doi.org/10.1371/journal.pone.0082673 |
work_keys_str_mv | AT loshyhching isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT libingjie isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT hungguochiuan isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT leihaiyan isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT litianwei isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT zhangjing isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT nagaminekenjiro isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT tsaishien isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT zuckermarkj isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness AT olesnickyludmilla isolationandcharacterizationoftwonovelbacteriaafipiacberiandmesorhizobiumhominisfrombloodofapatientafflictedwithfatalpulmonaryillness |