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Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers
Programs for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explore...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867518/ https://www.ncbi.nlm.nih.gov/pubmed/24367701 http://dx.doi.org/10.1371/journal.pone.0084917 |
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author | Pereira, Nátalli Zanete Cardoso, Elaine Cristina Oliveira, Luanda Mara da Silva de Lima, Josenilson Feitosa Branco, Anna Cláudia Calvielli Castelo Ruocco, Rosa Maria de Souza Aveiro Zugaib, Marcelo de Oliveira Filho, João Bosco Duarte, Alberto José da Silva Sato, Maria Notomi |
author_facet | Pereira, Nátalli Zanete Cardoso, Elaine Cristina Oliveira, Luanda Mara da Silva de Lima, Josenilson Feitosa Branco, Anna Cláudia Calvielli Castelo Ruocco, Rosa Maria de Souza Aveiro Zugaib, Marcelo de Oliveira Filho, João Bosco Duarte, Alberto José da Silva Sato, Maria Notomi |
author_sort | Pereira, Nátalli Zanete |
collection | PubMed |
description | Programs for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy (ART) in pregnancy (n=23) and in cord blood (CB) (n=16), placental tissues (n=10-13) and colostrum (n=5-6) samples and compared them to expression in samples from uninfected (UN) pregnant mothers (n=21). Mononuclear cells (MNCs) were prepared from maternal and CB samples following deliveries by cesarean section. Maternal (decidua) and fetal (chorionic villus) placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G (A3G), A3F, tripartite motif family-5α (TRIM-5α), TRIM-22, myxovirus resistance protein A (MxA), stimulator of interferon (IFN) genes (STING) and IFN-β, compared with the levels detected in uninfected (UN) mother-CB pairs. Moreover, A3G transcript and protein levels and α-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5α protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected (UN) mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-β mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection. |
format | Online Article Text |
id | pubmed-3867518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38675182013-12-23 Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers Pereira, Nátalli Zanete Cardoso, Elaine Cristina Oliveira, Luanda Mara da Silva de Lima, Josenilson Feitosa Branco, Anna Cláudia Calvielli Castelo Ruocco, Rosa Maria de Souza Aveiro Zugaib, Marcelo de Oliveira Filho, João Bosco Duarte, Alberto José da Silva Sato, Maria Notomi PLoS One Research Article Programs for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy (ART) in pregnancy (n=23) and in cord blood (CB) (n=16), placental tissues (n=10-13) and colostrum (n=5-6) samples and compared them to expression in samples from uninfected (UN) pregnant mothers (n=21). Mononuclear cells (MNCs) were prepared from maternal and CB samples following deliveries by cesarean section. Maternal (decidua) and fetal (chorionic villus) placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G (A3G), A3F, tripartite motif family-5α (TRIM-5α), TRIM-22, myxovirus resistance protein A (MxA), stimulator of interferon (IFN) genes (STING) and IFN-β, compared with the levels detected in uninfected (UN) mother-CB pairs. Moreover, A3G transcript and protein levels and α-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5α protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected (UN) mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-β mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection. Public Library of Science 2013-12-18 /pmc/articles/PMC3867518/ /pubmed/24367701 http://dx.doi.org/10.1371/journal.pone.0084917 Text en © 2013 Pereira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pereira, Nátalli Zanete Cardoso, Elaine Cristina Oliveira, Luanda Mara da Silva de Lima, Josenilson Feitosa Branco, Anna Cláudia Calvielli Castelo Ruocco, Rosa Maria de Souza Aveiro Zugaib, Marcelo de Oliveira Filho, João Bosco Duarte, Alberto José da Silva Sato, Maria Notomi Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title_full | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title_fullStr | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title_full_unstemmed | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title_short | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
title_sort | upregulation of innate antiviral restricting factor expression in the cord blood and decidual tissue of hiv-infected mothers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867518/ https://www.ncbi.nlm.nih.gov/pubmed/24367701 http://dx.doi.org/10.1371/journal.pone.0084917 |
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