Molecular characterization of Bacillus thuringiensis using rep-PCR

The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups w...

Descripción completa

Detalles Bibliográficos
Autores principales: da Silva, Rosane Bezerra, Valicente, Fernando Hercos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867629/
https://www.ncbi.nlm.nih.gov/pubmed/24363981
http://dx.doi.org/10.1186/2193-1801-2-641
_version_ 1782296330682499072
author da Silva, Rosane Bezerra
Valicente, Fernando Hercos
author_facet da Silva, Rosane Bezerra
Valicente, Fernando Hercos
author_sort da Silva, Rosane Bezerra
collection PubMed
description The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolates, 33,8% of the isolates were distributed in other groups and 24,6% did not formed distinct group. 53,2% of the isolates from Embrapa are in the group 1, and 29,8% of the isolates are distributed in other groups. Bt strains from USDA and Institute Pasteur showed more variability.
format Online
Article
Text
id pubmed-3867629
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-38676292013-12-20 Molecular characterization of Bacillus thuringiensis using rep-PCR da Silva, Rosane Bezerra Valicente, Fernando Hercos Springerplus Research The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolates, 33,8% of the isolates were distributed in other groups and 24,6% did not formed distinct group. 53,2% of the isolates from Embrapa are in the group 1, and 29,8% of the isolates are distributed in other groups. Bt strains from USDA and Institute Pasteur showed more variability. Springer International Publishing 2013-11-29 /pmc/articles/PMC3867629/ /pubmed/24363981 http://dx.doi.org/10.1186/2193-1801-2-641 Text en © da Silva and Valicente; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
da Silva, Rosane Bezerra
Valicente, Fernando Hercos
Molecular characterization of Bacillus thuringiensis using rep-PCR
title Molecular characterization of Bacillus thuringiensis using rep-PCR
title_full Molecular characterization of Bacillus thuringiensis using rep-PCR
title_fullStr Molecular characterization of Bacillus thuringiensis using rep-PCR
title_full_unstemmed Molecular characterization of Bacillus thuringiensis using rep-PCR
title_short Molecular characterization of Bacillus thuringiensis using rep-PCR
title_sort molecular characterization of bacillus thuringiensis using rep-pcr
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867629/
https://www.ncbi.nlm.nih.gov/pubmed/24363981
http://dx.doi.org/10.1186/2193-1801-2-641
work_keys_str_mv AT dasilvarosanebezerra molecularcharacterizationofbacillusthuringiensisusingreppcr
AT valicentefernandohercos molecularcharacterizationofbacillusthuringiensisusingreppcr

Ejemplares similares