An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications

Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high...

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Autores principales: Devi, Khumallambam Devala, Punyarani, Kshetrimayum, Singh, Nandeibam Samarjit, Devi, Huidrom Sunitibala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867630/
https://www.ncbi.nlm.nih.gov/pubmed/24363983
http://dx.doi.org/10.1186/2193-1801-2-669
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author Devi, Khumallambam Devala
Punyarani, Kshetrimayum
Singh, Nandeibam Samarjit
Devi, Huidrom Sunitibala
author_facet Devi, Khumallambam Devala
Punyarani, Kshetrimayum
Singh, Nandeibam Samarjit
Devi, Huidrom Sunitibala
author_sort Devi, Khumallambam Devala
collection PubMed
description Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses.
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spelling pubmed-38676302013-12-20 An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications Devi, Khumallambam Devala Punyarani, Kshetrimayum Singh, Nandeibam Samarjit Devi, Huidrom Sunitibala Springerplus Research Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses. Springer International Publishing 2013-12-13 /pmc/articles/PMC3867630/ /pubmed/24363983 http://dx.doi.org/10.1186/2193-1801-2-669 Text en © Devi et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Devi, Khumallambam Devala
Punyarani, Kshetrimayum
Singh, Nandeibam Samarjit
Devi, Huidrom Sunitibala
An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title_full An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title_fullStr An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title_full_unstemmed An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title_short An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications
title_sort efficient protocol for total dna extraction from the members of order zingiberales- suitable for diverse pcr based downstream applications
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867630/
https://www.ncbi.nlm.nih.gov/pubmed/24363983
http://dx.doi.org/10.1186/2193-1801-2-669
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