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Cloning, expression of b-1,3-1,4 glucanase from Bacillus subtilis SU40 and the effect of calcium ion on the stability of recombinant enzyme: in vitro and in silico analysis

A new glucanolytic bacterial strain, SU40 was isolated, and identified as Bacillus subtilis on the basis of 16S rRNA sequence homology and phylogenetic tree analysis. The gene encoding β-1,3-1,4-glucanase was delineated, cloned into pET 28a+ vector and heterologously overexpressed in Escherichia col...

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Detalles Bibliográficos
Autores principales:	Masilamani, Revathi, Sharma, Om Prakash, Muthuvel, Suresh Kumar, Natarajan, Sakthivel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Biomedical Informatics 2013
Materias:	Hypothesis
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867647/ https://www.ncbi.nlm.nih.gov/pubmed/24391357 http://dx.doi.org/10.6026/97320630009958

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