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Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats
BACKGROUND: Osteoporosis is characterized by low bone mass, bone fragility and increased susceptibility to fracture. Fracture healing in osteoporosis is delayed and rates of implant failure are high with few biological treatment options available. This study aimed to determine whether a single dose...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868133/ https://www.ncbi.nlm.nih.gov/pubmed/24379457 http://dx.doi.org/10.4103/0019-5413.121569 |
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author | Diwan, Ashish D Leong, Anthony Appleyard, Richard Bhargav, Divya Fang, Zhi Ming Wei, Aiqun |
author_facet | Diwan, Ashish D Leong, Anthony Appleyard, Richard Bhargav, Divya Fang, Zhi Ming Wei, Aiqun |
author_sort | Diwan, Ashish D |
collection | PubMed |
description | BACKGROUND: Osteoporosis is characterized by low bone mass, bone fragility and increased susceptibility to fracture. Fracture healing in osteoporosis is delayed and rates of implant failure are high with few biological treatment options available. This study aimed to determine whether a single dose of bone morphogenetic protein-7 (BMP-7) in a collagen/carboxy-methyl cellulose (CMC) composite enhanced fracture healing in an osteoporotic rat model. MATERIALS AND METHODS: An open femoral midshaft osteotomy was performed in female rats 3 months post-ovarectomy. Rats were randomized to receive either BMP-7 composite (n = 30) or composite alone (n = 30) at the fracture site during surgery. Thereafter calluses were collected on days 12, 20 and 31. Callus cross-sectional area, bone mineral density, biomechanical stiffness and maximum torque, radiographic bony union and histological callus maturity were evaluated at each time point. RESULTS: There were statistically significant increases in bone mineral density and callus cross-section area at all time points in the BMP-7 group as compared to controls and biomechanical readings showed stronger bones at day 31 in the BMP-7 group. Histological and radiographic evaluation indicated significant acceleration of bony union in the BMP-7 group as compared to controls. CONCLUSION: This study demonstrated that BMP-7 accelerates fracture healing in an oestrogen-deficient environment in a rat femoral fracture healing model to scientific relevance level I. The use of BMP-7 composite could offer orthopedic surgeons an advantage over oestrogen therapy, enhancing osteoporotic fracture healing with a single, locally applied dose at the time of surgery, potentially overcoming delays in healing caused by the osteoporotic state. |
format | Online Article Text |
id | pubmed-3868133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38681332013-12-30 Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats Diwan, Ashish D Leong, Anthony Appleyard, Richard Bhargav, Divya Fang, Zhi Ming Wei, Aiqun Indian J Orthop Original Article BACKGROUND: Osteoporosis is characterized by low bone mass, bone fragility and increased susceptibility to fracture. Fracture healing in osteoporosis is delayed and rates of implant failure are high with few biological treatment options available. This study aimed to determine whether a single dose of bone morphogenetic protein-7 (BMP-7) in a collagen/carboxy-methyl cellulose (CMC) composite enhanced fracture healing in an osteoporotic rat model. MATERIALS AND METHODS: An open femoral midshaft osteotomy was performed in female rats 3 months post-ovarectomy. Rats were randomized to receive either BMP-7 composite (n = 30) or composite alone (n = 30) at the fracture site during surgery. Thereafter calluses were collected on days 12, 20 and 31. Callus cross-sectional area, bone mineral density, biomechanical stiffness and maximum torque, radiographic bony union and histological callus maturity were evaluated at each time point. RESULTS: There were statistically significant increases in bone mineral density and callus cross-section area at all time points in the BMP-7 group as compared to controls and biomechanical readings showed stronger bones at day 31 in the BMP-7 group. Histological and radiographic evaluation indicated significant acceleration of bony union in the BMP-7 group as compared to controls. CONCLUSION: This study demonstrated that BMP-7 accelerates fracture healing in an oestrogen-deficient environment in a rat femoral fracture healing model to scientific relevance level I. The use of BMP-7 composite could offer orthopedic surgeons an advantage over oestrogen therapy, enhancing osteoporotic fracture healing with a single, locally applied dose at the time of surgery, potentially overcoming delays in healing caused by the osteoporotic state. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3868133/ /pubmed/24379457 http://dx.doi.org/10.4103/0019-5413.121569 Text en Copyright: © Indian Journal of Orthopaedics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Diwan, Ashish D Leong, Anthony Appleyard, Richard Bhargav, Divya Fang, Zhi Ming Wei, Aiqun Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title | Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title_full | Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title_fullStr | Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title_full_unstemmed | Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title_short | Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
title_sort | bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868133/ https://www.ncbi.nlm.nih.gov/pubmed/24379457 http://dx.doi.org/10.4103/0019-5413.121569 |
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