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Characterization of microRNA expression profiles in patients with intervertebral disc degeneration

Intervertebral disc degeneration (IDD) is associated with lower back pain and is a global burden with severe healthcare and socioeconomic consequences. However, the underlying mechanisms of IDD remain largely unelucidated. Accumulating evidence has indicasted that newly defined gene regulators, micr...

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Autores principales: ZHAO, BO, YU, QIANG, LI, HAOPENG, GUO, XIONG, HE, XIJING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868557/
https://www.ncbi.nlm.nih.gov/pubmed/24173697
http://dx.doi.org/10.3892/ijmm.2013.1543
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author ZHAO, BO
YU, QIANG
LI, HAOPENG
GUO, XIONG
HE, XIJING
author_facet ZHAO, BO
YU, QIANG
LI, HAOPENG
GUO, XIONG
HE, XIJING
author_sort ZHAO, BO
collection PubMed
description Intervertebral disc degeneration (IDD) is associated with lower back pain and is a global burden with severe healthcare and socioeconomic consequences. However, the underlying mechanisms of IDD remain largely unelucidated. Accumulating evidence has indicasted that newly defined gene regulators, microRNAs (miRNAs), play a vital role in neurodegenerative, pathophysiological and certain reproductive disorders. To characterize the differential miRNA expression profiles between IDD and spinal cord injury, specimens from 3 patients with IDD and 3 with spinal cord injury were selected for microarray analysis. Total RNA from these 6 specimens was extracted and subjected to global miRNA expression analysis using the Exiqon miRCURY™ LNA Array (v.16.0). The microarray data were then validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, bioinformatics analysis was performed to investigate the dysregulated miRNA target genes and signaling pathways involved. Among the miRNAs analyzed, 25 miRNAs were found to be upregulated and 26 were found to be downregulated in the IDD group compared with the spinal cord injury group. The qRT-PCR results validated the microarray data. Bioinformatics analysis indicated that the signaling pathways most likely to be controlled by these miRNAs were the phosphoinositide 3-kinase (PI3K)-Akt, mitogen-activated protein kinase (MAPK), epidermal growth factor receptor (EGFR; ErbB) and Wnt pathways. Our results demonstrated that the miRNA expression in patients with IDD differed significantly from that in patients who sustained injury to the intervertebral disc. Our data indicate that the dysregulated miRNAs control the signaling pathways important for the maintenance of IDD. Further studies on miRNA target gene identification and biological functions may address the specific regulatory mechanisms of miRNAs in IDD, and may provide valuable insight into the diagnosis and treatment of IDD.
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spelling pubmed-38685572013-12-20 Characterization of microRNA expression profiles in patients with intervertebral disc degeneration ZHAO, BO YU, QIANG LI, HAOPENG GUO, XIONG HE, XIJING Int J Mol Med Articles Intervertebral disc degeneration (IDD) is associated with lower back pain and is a global burden with severe healthcare and socioeconomic consequences. However, the underlying mechanisms of IDD remain largely unelucidated. Accumulating evidence has indicasted that newly defined gene regulators, microRNAs (miRNAs), play a vital role in neurodegenerative, pathophysiological and certain reproductive disorders. To characterize the differential miRNA expression profiles between IDD and spinal cord injury, specimens from 3 patients with IDD and 3 with spinal cord injury were selected for microarray analysis. Total RNA from these 6 specimens was extracted and subjected to global miRNA expression analysis using the Exiqon miRCURY™ LNA Array (v.16.0). The microarray data were then validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, bioinformatics analysis was performed to investigate the dysregulated miRNA target genes and signaling pathways involved. Among the miRNAs analyzed, 25 miRNAs were found to be upregulated and 26 were found to be downregulated in the IDD group compared with the spinal cord injury group. The qRT-PCR results validated the microarray data. Bioinformatics analysis indicated that the signaling pathways most likely to be controlled by these miRNAs were the phosphoinositide 3-kinase (PI3K)-Akt, mitogen-activated protein kinase (MAPK), epidermal growth factor receptor (EGFR; ErbB) and Wnt pathways. Our results demonstrated that the miRNA expression in patients with IDD differed significantly from that in patients who sustained injury to the intervertebral disc. Our data indicate that the dysregulated miRNAs control the signaling pathways important for the maintenance of IDD. Further studies on miRNA target gene identification and biological functions may address the specific regulatory mechanisms of miRNAs in IDD, and may provide valuable insight into the diagnosis and treatment of IDD. D.A. Spandidos 2014-01 2013-10-30 /pmc/articles/PMC3868557/ /pubmed/24173697 http://dx.doi.org/10.3892/ijmm.2013.1543 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHAO, BO
YU, QIANG
LI, HAOPENG
GUO, XIONG
HE, XIJING
Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title_full Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title_fullStr Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title_full_unstemmed Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title_short Characterization of microRNA expression profiles in patients with intervertebral disc degeneration
title_sort characterization of microrna expression profiles in patients with intervertebral disc degeneration
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868557/
https://www.ncbi.nlm.nih.gov/pubmed/24173697
http://dx.doi.org/10.3892/ijmm.2013.1543
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