Cargando…

Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes

BACKGROUND: Attractive toxic sugar bait (ATSB) sprayed onto vegetation has been successful in controlling Anopheles mosquitoes outdoors. Indoor application of ATSB has yet to be explored. The purpose of this study was to determine whether ATSB stations positioned indoors have the potential to kill h...

Descripción completa

Detalles Bibliográficos
Autores principales: Stewart, Zachary P., Oxborough, Richard M., Tungu, Patrick K., Kirby, Matthew J., Rowland, Mark W., Irish, Seth R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868566/
https://www.ncbi.nlm.nih.gov/pubmed/24367638
http://dx.doi.org/10.1371/journal.pone.0084168
_version_ 1782296471592239104
author Stewart, Zachary P.
Oxborough, Richard M.
Tungu, Patrick K.
Kirby, Matthew J.
Rowland, Mark W.
Irish, Seth R.
author_facet Stewart, Zachary P.
Oxborough, Richard M.
Tungu, Patrick K.
Kirby, Matthew J.
Rowland, Mark W.
Irish, Seth R.
author_sort Stewart, Zachary P.
collection PubMed
description BACKGROUND: Attractive toxic sugar bait (ATSB) sprayed onto vegetation has been successful in controlling Anopheles mosquitoes outdoors. Indoor application of ATSB has yet to be explored. The purpose of this study was to determine whether ATSB stations positioned indoors have the potential to kill host-seeking mosquitoes and constitute a new approach to control of mosquito-borne diseases. METHODS: Insecticides were mixed with dyed sugar solution and tested as toxic baits against Anopheles arabiensis, An. Gambiae s.s. and Culex quinquefasciatus in feeding bioassay tests to identify suitable attractant-insecticide combinations. The most promising ATSB candidates were then trialed in experimental huts in Moshi, Tanzania. ATSB stations were hung in huts next to untreated mosquito nets occupied by human volunteers. The proportions of mosquitoes killed in huts with ATSB treatments relative to huts with non-insecticide control treatments huts were recorded, noting evidence of dye in mosquito abdomens. RESULTS: In feeding bioassays, chlorfenapyr 0.5% v/v, boric acid 2% w/v, and tolfenpyrad 1% v/v, mixed in a guava juice-based bait, each killed more than 90% of pyrethroid-susceptible An. Gambiae s.s. and pyrethroid-resistant An. arabiensis and Cx. quinquefasciatus. In the hut trial, mortality rates of the three ATSB treatments ranged from 41-48% against An. arabiensis and 36-43% against Cx. quinquefasciatus and all were significantly greater than the control mortalities: 18% for An. arabiensis, 7% for Cx. quinquefasciatus (p<0.05). Mortality rates with ATSB were comparable to those with long lasting insecticidal nets previously tested against the same species in this area. CONCLUSIONS: Indoor ATSB shows promise as a supplement to mosquito nets for controlling mosquitoes. Indoor ATSB constitute a novel application method for insecticide classes that act as stomach poisons and have not hitherto been exploited for mosquito control. Combined with LLIN, indoor use of ATSB has the potential to serve as a strategy for managing insecticide resistance.
format Online
Article
Text
id pubmed-3868566
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38685662013-12-23 Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes Stewart, Zachary P. Oxborough, Richard M. Tungu, Patrick K. Kirby, Matthew J. Rowland, Mark W. Irish, Seth R. PLoS One Research Article BACKGROUND: Attractive toxic sugar bait (ATSB) sprayed onto vegetation has been successful in controlling Anopheles mosquitoes outdoors. Indoor application of ATSB has yet to be explored. The purpose of this study was to determine whether ATSB stations positioned indoors have the potential to kill host-seeking mosquitoes and constitute a new approach to control of mosquito-borne diseases. METHODS: Insecticides were mixed with dyed sugar solution and tested as toxic baits against Anopheles arabiensis, An. Gambiae s.s. and Culex quinquefasciatus in feeding bioassay tests to identify suitable attractant-insecticide combinations. The most promising ATSB candidates were then trialed in experimental huts in Moshi, Tanzania. ATSB stations were hung in huts next to untreated mosquito nets occupied by human volunteers. The proportions of mosquitoes killed in huts with ATSB treatments relative to huts with non-insecticide control treatments huts were recorded, noting evidence of dye in mosquito abdomens. RESULTS: In feeding bioassays, chlorfenapyr 0.5% v/v, boric acid 2% w/v, and tolfenpyrad 1% v/v, mixed in a guava juice-based bait, each killed more than 90% of pyrethroid-susceptible An. Gambiae s.s. and pyrethroid-resistant An. arabiensis and Cx. quinquefasciatus. In the hut trial, mortality rates of the three ATSB treatments ranged from 41-48% against An. arabiensis and 36-43% against Cx. quinquefasciatus and all were significantly greater than the control mortalities: 18% for An. arabiensis, 7% for Cx. quinquefasciatus (p<0.05). Mortality rates with ATSB were comparable to those with long lasting insecticidal nets previously tested against the same species in this area. CONCLUSIONS: Indoor ATSB shows promise as a supplement to mosquito nets for controlling mosquitoes. Indoor ATSB constitute a novel application method for insecticide classes that act as stomach poisons and have not hitherto been exploited for mosquito control. Combined with LLIN, indoor use of ATSB has the potential to serve as a strategy for managing insecticide resistance. Public Library of Science 2013-12-19 /pmc/articles/PMC3868566/ /pubmed/24367638 http://dx.doi.org/10.1371/journal.pone.0084168 Text en © 2013 Stewart et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Stewart, Zachary P.
Oxborough, Richard M.
Tungu, Patrick K.
Kirby, Matthew J.
Rowland, Mark W.
Irish, Seth R.
Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title_full Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title_fullStr Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title_full_unstemmed Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title_short Indoor Application of Attractive Toxic Sugar Bait (ATSB) in Combination with Mosquito Nets for Control of Pyrethroid-Resistant Mosquitoes
title_sort indoor application of attractive toxic sugar bait (atsb) in combination with mosquito nets for control of pyrethroid-resistant mosquitoes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868566/
https://www.ncbi.nlm.nih.gov/pubmed/24367638
http://dx.doi.org/10.1371/journal.pone.0084168
work_keys_str_mv AT stewartzacharyp indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes
AT oxboroughrichardm indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes
AT tungupatrickk indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes
AT kirbymatthewj indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes
AT rowlandmarkw indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes
AT irishsethr indoorapplicationofattractivetoxicsugarbaitatsbincombinationwithmosquitonetsforcontrolofpyrethroidresistantmosquitoes