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In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection

CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs...

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Autores principales: Martirosyan, Anna, Von Bargen, Kristine, Arce Gorvel, Vilma, Zhao, Weidong, Hanniffy, Sean, Bonnardel, Johnny, Méresse, Stéphane, Gorvel, Jean-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868576/
https://www.ncbi.nlm.nih.gov/pubmed/24367519
http://dx.doi.org/10.1371/journal.pone.0082508
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author Martirosyan, Anna
Von Bargen, Kristine
Arce Gorvel, Vilma
Zhao, Weidong
Hanniffy, Sean
Bonnardel, Johnny
Méresse, Stéphane
Gorvel, Jean-Pierre
author_facet Martirosyan, Anna
Von Bargen, Kristine
Arce Gorvel, Vilma
Zhao, Weidong
Hanniffy, Sean
Bonnardel, Johnny
Méresse, Stéphane
Gorvel, Jean-Pierre
author_sort Martirosyan, Anna
collection PubMed
description CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs express granzyme B and exhibit immunophenotypic features consistent with fully differentiated T cells. They express CD25, CD44, CD62L ,CD43 molecules at their surface and produce IFN-γ. Moreover, these cells express neither the co-stimulatory molecule CD27 nor the memory T cell marker CD127. We show here that CD4(+) CTLs are capable of cytolytic action against Brucella-infected antigen presenting cells (APC) but not against Mycobacterium-infected APC. Cytotoxic CD4(+) T cell population appears at early stages of the infection concomitantly with high levels of IFN-γ and granzyme B expression. CD4(+) CTLs represent a so far uncharacterized immune cell sub-type triggered by early immune responses upon Brucella abortus infection.
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spelling pubmed-38685762013-12-23 In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection Martirosyan, Anna Von Bargen, Kristine Arce Gorvel, Vilma Zhao, Weidong Hanniffy, Sean Bonnardel, Johnny Méresse, Stéphane Gorvel, Jean-Pierre PLoS One Research Article CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs express granzyme B and exhibit immunophenotypic features consistent with fully differentiated T cells. They express CD25, CD44, CD62L ,CD43 molecules at their surface and produce IFN-γ. Moreover, these cells express neither the co-stimulatory molecule CD27 nor the memory T cell marker CD127. We show here that CD4(+) CTLs are capable of cytolytic action against Brucella-infected antigen presenting cells (APC) but not against Mycobacterium-infected APC. Cytotoxic CD4(+) T cell population appears at early stages of the infection concomitantly with high levels of IFN-γ and granzyme B expression. CD4(+) CTLs represent a so far uncharacterized immune cell sub-type triggered by early immune responses upon Brucella abortus infection. Public Library of Science 2013-12-19 /pmc/articles/PMC3868576/ /pubmed/24367519 http://dx.doi.org/10.1371/journal.pone.0082508 Text en © 2013 Martirosyan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Martirosyan, Anna
Von Bargen, Kristine
Arce Gorvel, Vilma
Zhao, Weidong
Hanniffy, Sean
Bonnardel, Johnny
Méresse, Stéphane
Gorvel, Jean-Pierre
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title_full In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title_fullStr In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title_full_unstemmed In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title_short In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
title_sort in vivo identification and characterization of cd4(+) cytotoxic t cells induced by virulent brucella abortus infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868576/
https://www.ncbi.nlm.nih.gov/pubmed/24367519
http://dx.doi.org/10.1371/journal.pone.0082508
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