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In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection
CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868576/ https://www.ncbi.nlm.nih.gov/pubmed/24367519 http://dx.doi.org/10.1371/journal.pone.0082508 |
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author | Martirosyan, Anna Von Bargen, Kristine Arce Gorvel, Vilma Zhao, Weidong Hanniffy, Sean Bonnardel, Johnny Méresse, Stéphane Gorvel, Jean-Pierre |
author_facet | Martirosyan, Anna Von Bargen, Kristine Arce Gorvel, Vilma Zhao, Weidong Hanniffy, Sean Bonnardel, Johnny Méresse, Stéphane Gorvel, Jean-Pierre |
author_sort | Martirosyan, Anna |
collection | PubMed |
description | CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs express granzyme B and exhibit immunophenotypic features consistent with fully differentiated T cells. They express CD25, CD44, CD62L ,CD43 molecules at their surface and produce IFN-γ. Moreover, these cells express neither the co-stimulatory molecule CD27 nor the memory T cell marker CD127. We show here that CD4(+) CTLs are capable of cytolytic action against Brucella-infected antigen presenting cells (APC) but not against Mycobacterium-infected APC. Cytotoxic CD4(+) T cell population appears at early stages of the infection concomitantly with high levels of IFN-γ and granzyme B expression. CD4(+) CTLs represent a so far uncharacterized immune cell sub-type triggered by early immune responses upon Brucella abortus infection. |
format | Online Article Text |
id | pubmed-3868576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38685762013-12-23 In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection Martirosyan, Anna Von Bargen, Kristine Arce Gorvel, Vilma Zhao, Weidong Hanniffy, Sean Bonnardel, Johnny Méresse, Stéphane Gorvel, Jean-Pierre PLoS One Research Article CD4(+) T cells display a variety of helper functions necessary for an efficient adaptive immune response against bacterial invaders. This work reports the in vivo identification and characterization of murine cytotoxic CD4(+) T cells (CD4(+) CTL) during Brucella abortus infection. These CD4(+) CTLs express granzyme B and exhibit immunophenotypic features consistent with fully differentiated T cells. They express CD25, CD44, CD62L ,CD43 molecules at their surface and produce IFN-γ. Moreover, these cells express neither the co-stimulatory molecule CD27 nor the memory T cell marker CD127. We show here that CD4(+) CTLs are capable of cytolytic action against Brucella-infected antigen presenting cells (APC) but not against Mycobacterium-infected APC. Cytotoxic CD4(+) T cell population appears at early stages of the infection concomitantly with high levels of IFN-γ and granzyme B expression. CD4(+) CTLs represent a so far uncharacterized immune cell sub-type triggered by early immune responses upon Brucella abortus infection. Public Library of Science 2013-12-19 /pmc/articles/PMC3868576/ /pubmed/24367519 http://dx.doi.org/10.1371/journal.pone.0082508 Text en © 2013 Martirosyan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martirosyan, Anna Von Bargen, Kristine Arce Gorvel, Vilma Zhao, Weidong Hanniffy, Sean Bonnardel, Johnny Méresse, Stéphane Gorvel, Jean-Pierre In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title |
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title_full |
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title_fullStr |
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title_full_unstemmed |
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title_short |
In Vivo Identification and Characterization of CD4(+) Cytotoxic T Cells Induced by Virulent Brucella abortus Infection |
title_sort | in vivo identification and characterization of cd4(+) cytotoxic t cells induced by virulent brucella abortus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868576/ https://www.ncbi.nlm.nih.gov/pubmed/24367519 http://dx.doi.org/10.1371/journal.pone.0082508 |
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