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Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na (v)1.5 expressed in HEK293 cells
Background: The pore-forming subunit of the cardiac sodium channel, Na (v)1.5, has been previously found to be mutated in genetically determined arrhythmias. Na (v)1.5 associates with many proteins that regulate its function and cellular localisation. In order to identify more in situ Na (v)1.5 inte...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869486/ https://www.ncbi.nlm.nih.gov/pubmed/24555036 http://dx.doi.org/10.12688/f1000research.2-48.v2 |
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author | Reinhard, Katja Rougier, Jean-Sébastien Ogrodnik, Jakob Abriel, Hugues |
author_facet | Reinhard, Katja Rougier, Jean-Sébastien Ogrodnik, Jakob Abriel, Hugues |
author_sort | Reinhard, Katja |
collection | PubMed |
description | Background: The pore-forming subunit of the cardiac sodium channel, Na (v)1.5, has been previously found to be mutated in genetically determined arrhythmias. Na (v)1.5 associates with many proteins that regulate its function and cellular localisation. In order to identify more in situ Na (v)1.5 interacting proteins, genetically-modified mice with a high-affinity epitope in the sequence of Na (v)1.5 can be generated. Methods: In this short study, we (1) compared the biophysical properties of the sodium current (I (Na)) generated by the mouse Na (v)1.5 (mNa (v)1.5) and human Na (v)1.5 (hNa (v)1.5) constructs that were expressed in HEK293 cells, and (2) investigated the possible alterations of the biophysical properties of the human Na (v)1.5 construct that was modified with specific epitopes. Results: The biophysical properties of mNa (v)1.5 were similar to the human homolog. Addition of epitopes either up-stream of the N-terminus of hNa (v)1.5 or in the extracellular loop between the S5 and S6 transmembrane segments of domain 1, significantly decreased the amount of I (Na) and slightly altered its biophysical properties. Adding green fluorescent protein (GFP) to the N-terminus did not modify any of the measured biophysical properties of hNa (v)1.5. Conclusions: These findings have to be taken into account when planning to generate genetically-modified mouse models that harbour specific epitopes in the gene encoding mNa (v)1.5. |
format | Online Article Text |
id | pubmed-3869486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-38694862013-12-27 Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na (v)1.5 expressed in HEK293 cells Reinhard, Katja Rougier, Jean-Sébastien Ogrodnik, Jakob Abriel, Hugues F1000Res Research Article Background: The pore-forming subunit of the cardiac sodium channel, Na (v)1.5, has been previously found to be mutated in genetically determined arrhythmias. Na (v)1.5 associates with many proteins that regulate its function and cellular localisation. In order to identify more in situ Na (v)1.5 interacting proteins, genetically-modified mice with a high-affinity epitope in the sequence of Na (v)1.5 can be generated. Methods: In this short study, we (1) compared the biophysical properties of the sodium current (I (Na)) generated by the mouse Na (v)1.5 (mNa (v)1.5) and human Na (v)1.5 (hNa (v)1.5) constructs that were expressed in HEK293 cells, and (2) investigated the possible alterations of the biophysical properties of the human Na (v)1.5 construct that was modified with specific epitopes. Results: The biophysical properties of mNa (v)1.5 were similar to the human homolog. Addition of epitopes either up-stream of the N-terminus of hNa (v)1.5 or in the extracellular loop between the S5 and S6 transmembrane segments of domain 1, significantly decreased the amount of I (Na) and slightly altered its biophysical properties. Adding green fluorescent protein (GFP) to the N-terminus did not modify any of the measured biophysical properties of hNa (v)1.5. Conclusions: These findings have to be taken into account when planning to generate genetically-modified mouse models that harbour specific epitopes in the gene encoding mNa (v)1.5. F1000Research 2013-04-05 /pmc/articles/PMC3869486/ /pubmed/24555036 http://dx.doi.org/10.12688/f1000research.2-48.v2 Text en Copyright: © 2013 Reinhard K et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/publicdomain/zero/1.0/ Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication). |
spellingShingle | Research Article Reinhard, Katja Rougier, Jean-Sébastien Ogrodnik, Jakob Abriel, Hugues Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na (v)1.5 expressed in HEK293 cells |
title | Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na
(v)1.5 expressed in HEK293 cells |
title_full | Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na
(v)1.5 expressed in HEK293 cells |
title_fullStr | Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na
(v)1.5 expressed in HEK293 cells |
title_full_unstemmed | Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na
(v)1.5 expressed in HEK293 cells |
title_short | Electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel Na
(v)1.5 expressed in HEK293 cells |
title_sort | electrophysiological properties of mouse and epitope-tagged human cardiac sodium channel na
(v)1.5 expressed in hek293 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869486/ https://www.ncbi.nlm.nih.gov/pubmed/24555036 http://dx.doi.org/10.12688/f1000research.2-48.v2 |
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