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Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation
The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different proces...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869556/ https://www.ncbi.nlm.nih.gov/pubmed/24363709 |
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author | Ghaffari, Mohammad Ali Shanaki, Mehrnoosh |
author_facet | Ghaffari, Mohammad Ali Shanaki, Mehrnoosh |
author_sort | Ghaffari, Mohammad Ali |
collection | PubMed |
description | The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL carbamylation may contribute to the increased risk of atherosclerosis in patients with kidney failure. The objective of this study was to investigate in vitro effects of flavonoids on LDL carbamylation. LDL was isolated from plasma using ultracentrifuge technique with a single step discontinuous gradient. Then, cyanate was added to LDL and LDL carbamylation level was estimated in absence and presence of flavonoids by a colorimetric method at 530 nm. The results of this study showed that a number of flavonoids including rutin, catechin, morin, myricetin, kaempferol, taxifolin, luteolin, naringin and quercetin decreased LDL carbamylation in a dose dependent manner. Also, it was demonstrated that these nutrients decreased electrophoretic mobility of carbamylated LDL. Based on the results obtained in this study, it is suggested that flavonoids are able to inhibit LDL carbamylation (probably by scavenging cyanate ions) and thus, may have a role in ameliorating atherosclerotic risk of patients with kidney failure. |
format | Online Article Text |
id | pubmed-3869556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38695562013-12-20 Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation Ghaffari, Mohammad Ali Shanaki, Mehrnoosh Iran J Pharm Res Original Article The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL carbamylation may contribute to the increased risk of atherosclerosis in patients with kidney failure. The objective of this study was to investigate in vitro effects of flavonoids on LDL carbamylation. LDL was isolated from plasma using ultracentrifuge technique with a single step discontinuous gradient. Then, cyanate was added to LDL and LDL carbamylation level was estimated in absence and presence of flavonoids by a colorimetric method at 530 nm. The results of this study showed that a number of flavonoids including rutin, catechin, morin, myricetin, kaempferol, taxifolin, luteolin, naringin and quercetin decreased LDL carbamylation in a dose dependent manner. Also, it was demonstrated that these nutrients decreased electrophoretic mobility of carbamylated LDL. Based on the results obtained in this study, it is suggested that flavonoids are able to inhibit LDL carbamylation (probably by scavenging cyanate ions) and thus, may have a role in ameliorating atherosclerotic risk of patients with kidney failure. Shaheed Beheshti University of Medical Sciences 2010 /pmc/articles/PMC3869556/ /pubmed/24363709 Text en © 2010 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ghaffari, Mohammad Ali Shanaki, Mehrnoosh Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title | Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title_full | Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title_fullStr | Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title_full_unstemmed | Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title_short | Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation |
title_sort | evalution of in vitro effect of flavonoids on human low-density lipoprotein carbamylation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869556/ https://www.ncbi.nlm.nih.gov/pubmed/24363709 |
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