Hepatoprotective Effect of Ficus carica Leaf Extract on Mice Intoxicated with Carbon Tetrachloride

Protective action of Ficus carica leaf ethanolic extract (obtained by maceration) was evaluated in an animal model of hepatotoxicity induced by carbon tetrachloride (CCl(4)). Male albino mice were divided into six groups. group I was normal control group; group II received olive oil (CCl(4) solvent), groups III-VI received CCl(4). After inducing hepatic damage, group III served as control for CCl(4); and groups IV- VI received different doses of Ficus carica ethanol extract (200, 400 and 800 mg/kg) prior to intoxication with CCl(4). Liver marker enzymes were assayed in serum. Sections of livers were observed under microscope for the histopathological changes. Levels of marker enzymes such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased significantly in CCl(4) treated mice (group III). In groups IV, V and VI, pre-treated with the plant extract and intoxicated with CCl(4), decreased activities of these two enzymes were observed. Also, pre-treatment with the extract in these groups resulted in less pronounced destruction of the liver architecture with no fibrosis and moderate inflammation was observed compared with group III. The present observations suggested that the treatment with Ficus carica leaf extract in dose of 200 mg/kg enhanced protection against CCl(4) induced hepatic damage.