Cargando…

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

Internal ribosome entry site (IRES) elements found in the 5′ untranslated region of mRNAs enable translation initiation in a cap-independent manner, thereby representing an alternative to cap-dependent translation in cell-free protein expression systems. However, IRES function is largely species-dep...

Descripción completa

Detalles Bibliográficos
Autores principales:	Brödel, Andreas K., Sonnabend, Andrei, Roberts, Lisa O., Stech, Marlitt, Wüstenhagen, Doreen A., Kubick, Stefan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869664/ https://www.ncbi.nlm.nih.gov/pubmed/24376523 http://dx.doi.org/10.1371/journal.pone.0082234

Ejemplares similares

High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format
por: Quast, Robert B., et al.
Publicado: (2016)

Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives
por: Thoring, Lena, et al.
Publicado: (2016)

Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids
por: Quast, Robert B., et al.
Publicado: (2016)

High-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates
por: Thoring, Lena, et al.
Publicado: (2017)

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells
por: Stech, Marlitt, et al.
Publicado: (2014)

Production of G protein‐coupled receptors in an insect‐based cell‐free system
por: Sonnabend, Andrei, et al.
Publicado: (2017)

Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments
por: Stech, Marlitt, et al.
Publicado: (2014)

A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies
por: Haueis, Lisa, et al.
Publicado: (2022)

Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus
por: Ramm, Franziska, et al.
Publicado: (2020)

Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus
por: Thoring, Lena, et al.
Publicado: (2019)

Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems
por: Dondapati, Srujan Kumar, et al.
Publicado: (2019)

Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems
por: Krebs, Simon K., et al.
Publicado: (2022)

Rapid One-Step Capturing of Native, Cell-Free Synthesized and Membrane-Embedded GLP-1R
por: Haueis, Lisa, et al.
Publicado: (2023)

Cell-Free Protein Synthesis: A Promising Option for Future Drug Development
por: Dondapati, Srujan Kumar, et al.
Publicado: (2020)

The Pore-Forming Hemolysin BL Enterotoxin from Bacillus cereus: Subunit Interactions in Cell-Free Systems
por: Ramm, Franziska, et al.
Publicado: (2021)

Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems
por: Dhandapani, Priyavathi, et al.
Publicado: (2021)

Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses
por: Zemella, Anne, et al.
Publicado: (2017)

A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality
por: Krebs, Simon K., et al.
Publicado: (2022)

Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates
por: Stech, M., et al.
Publicado: (2017)

Cell‐free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus
por: Dondapati, Srujan Kumar, et al.
Publicado: (2017)

Initiation of Translation in Bacteria by a Structured Eukaryotic IRES RNA
por: Colussi, Timothy M., et al.
Publicado: (2015)

Cell-free synthesis of human toll-like receptor 9 (TLR9): Optimization of synthesis conditions and functional analysis
por: Dondapati, Srujan Kumar, et al.
Publicado: (2019)

Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
por: Zemella, Anne, et al.
Publicado: (2018)

Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis
por: Wüstenhagen, Doreen A., et al.
Publicado: (2020)

Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems
por: Schloßhauer, Jeffrey L., et al.
Publicado: (2022)

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems
por: Zemella, Anne, et al.
Publicado: (2015)

Kinetics of CrPV and HCV IRES-mediated eukaryotic translation using single-molecule fluorescence microscopy
por: Bugaud, Olivier, et al.
Publicado: (2017)

Characterization of trh2 Harbouring Vibrio parahaemolyticus Strains Isolated in Germany
por: Bechlars, Silke, et al.
Publicado: (2015)

Oligomerization of the heteromeric γ-aminobutyric acid receptor GABA(B) in a eukaryotic cell-free system
por: Ullrich, Jessica, et al.
Publicado: (2022)

Small molecule inhibitors of IRES-mediated translation
por: Vaklavas, Christos, et al.
Publicado: (2015)

A novel mechanism of eukaryotic translation initiation that is neither m(7)G-cap-, nor IRES-dependent
por: Terenin, Ilya M., et al.
Publicado: (2013)

Glycoprotein Biosynthesis in a Eukaryote Lacking the Membrane Protein Rft1
por: Jelk, Jennifer, et al.
Publicado: (2013)

Human IRES Atlas: an integrative platform for studying IRES-driven translational regulation in humans
por: Yang, Tzu-Hsien, et al.
Publicado: (2021)

Evaluation of the Ion Channel Assembly in a Eukaryotic Cell-Free System Focusing on Two-Pore Domain Potassium Channels K(2P)
por: Ullrich, Jessica, et al.
Publicado: (2023)

PTBP1 promotes IRES-mediated translation of cyclin B1in cancer: PTBP1 promotes IRES-mediated translation of cyclin B1 in cancer
por: Fan, Xinyi, et al.
Publicado: (2022)

Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool
por: Schloßhauer, Jeffrey L., et al.
Publicado: (2023)

Hepatitis C virus NS4A inhibits cap-dependent and the viral IRES-mediated translation through interacting with eukaryotic elongation factor 1A
por: Kou, Yi-Hen, et al.
Publicado: (2006)

Insights into the Role of Yeast eIF2A in IRES-Mediated Translation
por: Reineke, Lucas C., et al.
Publicado: (2011)

Promotion of Viral IRES-Mediated Translation Initiation under Mild Hypothermia
por: Licursi, Maria, et al.
Publicado: (2015)

Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses
por: Abdullah, Sahibzada Waheed, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_icn9p5o68i36h8a72d9rl1nio2