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Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18
Human γδ T cells augment host defense against tumors and infections, and might have a therapeutic potential in immunotherapy. However, mechanism of γδ T cell proliferation is unclear, and therefore it is difficult to prepare sufficient numbers of γδ T cells for clinical immunotherapy. Recently, natu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869690/ https://www.ncbi.nlm.nih.gov/pubmed/24376549 http://dx.doi.org/10.1371/journal.pone.0082586 |
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author | Li, Wen Okuda, Akico Yamamoto, Hideyuki Yamanishi, Kyosuke Terada, Nobuyuki Yamanishi, Hiromichi Tanaka, Yoshimasa Okamura, Haruki |
author_facet | Li, Wen Okuda, Akico Yamamoto, Hideyuki Yamanishi, Kyosuke Terada, Nobuyuki Yamanishi, Hiromichi Tanaka, Yoshimasa Okamura, Haruki |
author_sort | Li, Wen |
collection | PubMed |
description | Human γδ T cells augment host defense against tumors and infections, and might have a therapeutic potential in immunotherapy. However, mechanism of γδ T cell proliferation is unclear, and therefore it is difficult to prepare sufficient numbers of γδ T cells for clinical immunotherapy. Recently, natural killer (NK)-like CD56(bright)CD11c(+) cells were shown to promote the proliferation of γδ T cells in an IL-18-dependent manner. In this study, we demonstrated that the NK-like CD56(bright)CD11c(+) cells could directly interact with γδ T cells to promote their sustained expansion, while conventional dendritic cells (DCs), IFN-α-induced DCs, plasmacytoid DCs or monocytes did not. We also examined the cellular mechanism underlying the regulation of CD56(bright)CD11c(+) cells. CD14(+) monocytes pre-incubated with IL-2/IL-18 formed intensive interactions with CD56(int)CD11c(+) cells to promote their differentiation to CD56(bright)CD11c(+) cells with helper function. The development of CD56(bright)CD11c(+) cells was suppressed in an IFN-α dependent manner. These results indicate that CD14(+) monocytes pretreated with IL-2/IL-18, but neither DCs nor monocytes, play a determining role on the development and proliferation of CD56(bright)CD11c(+) cells, which in turn modulate the expansion of γδ T cells. CD56(bright)CD11c(+) NK-like cells may be a novel target for immunotherapy utilizing γδ T cells, by overcoming the limitation of γδ T cells proliferation. |
format | Online Article Text |
id | pubmed-3869690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38696902013-12-27 Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 Li, Wen Okuda, Akico Yamamoto, Hideyuki Yamanishi, Kyosuke Terada, Nobuyuki Yamanishi, Hiromichi Tanaka, Yoshimasa Okamura, Haruki PLoS One Research Article Human γδ T cells augment host defense against tumors and infections, and might have a therapeutic potential in immunotherapy. However, mechanism of γδ T cell proliferation is unclear, and therefore it is difficult to prepare sufficient numbers of γδ T cells for clinical immunotherapy. Recently, natural killer (NK)-like CD56(bright)CD11c(+) cells were shown to promote the proliferation of γδ T cells in an IL-18-dependent manner. In this study, we demonstrated that the NK-like CD56(bright)CD11c(+) cells could directly interact with γδ T cells to promote their sustained expansion, while conventional dendritic cells (DCs), IFN-α-induced DCs, plasmacytoid DCs or monocytes did not. We also examined the cellular mechanism underlying the regulation of CD56(bright)CD11c(+) cells. CD14(+) monocytes pre-incubated with IL-2/IL-18 formed intensive interactions with CD56(int)CD11c(+) cells to promote their differentiation to CD56(bright)CD11c(+) cells with helper function. The development of CD56(bright)CD11c(+) cells was suppressed in an IFN-α dependent manner. These results indicate that CD14(+) monocytes pretreated with IL-2/IL-18, but neither DCs nor monocytes, play a determining role on the development and proliferation of CD56(bright)CD11c(+) cells, which in turn modulate the expansion of γδ T cells. CD56(bright)CD11c(+) NK-like cells may be a novel target for immunotherapy utilizing γδ T cells, by overcoming the limitation of γδ T cells proliferation. Public Library of Science 2013-12-20 /pmc/articles/PMC3869690/ /pubmed/24376549 http://dx.doi.org/10.1371/journal.pone.0082586 Text en © 2013 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Wen Okuda, Akico Yamamoto, Hideyuki Yamanishi, Kyosuke Terada, Nobuyuki Yamanishi, Hiromichi Tanaka, Yoshimasa Okamura, Haruki Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title | Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title_full | Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title_fullStr | Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title_full_unstemmed | Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title_short | Regulation of Development of CD56(bright)CD11c(+) NK-like Cells with Helper Function by IL-18 |
title_sort | regulation of development of cd56(bright)cd11c(+) nk-like cells with helper function by il-18 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869690/ https://www.ncbi.nlm.nih.gov/pubmed/24376549 http://dx.doi.org/10.1371/journal.pone.0082586 |
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