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TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection

Toll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2...

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Autores principales: Gao, Yanan, Chen, Lin, Hou, Min, Chen, Yingying, Ji, Minjun, Wu, Haiwei, Wu, Guanling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869711/
https://www.ncbi.nlm.nih.gov/pubmed/24376539
http://dx.doi.org/10.1371/journal.pone.0082480
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author Gao, Yanan
Chen, Lin
Hou, Min
Chen, Yingying
Ji, Minjun
Wu, Haiwei
Wu, Guanling
author_facet Gao, Yanan
Chen, Lin
Hou, Min
Chen, Yingying
Ji, Minjun
Wu, Haiwei
Wu, Guanling
author_sort Gao, Yanan
collection PubMed
description Toll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2(−/−)) mice compared with B6 mice. In Schistosoma japonicum chronic infection models, programmed death ligand 1 (PD-L1) and programmed death ligand 2 (PD-L2) expression as well as TLR2 expression gradually increased in B6 mice, while only PD-L2 expression significantly decreased in TLR2(−/−) mice. Meanwhile, Programmed Death 1(PD-1) expression on CD4(+)T cells was down-regulated in TLR2(−/−) mice after a large number of egg appeared. We also found that stimulation with schistosome antigens, especially SEA, could up-regulate PD-L2 expression on BMDCs in a TLR2-dependent manner in vitro. Schistosome antigens primed-BMDCs with impaired expression of TLR2 or PD-L2 could induce CD4(+)T cells to produce low level of IL-10 or high level of IFN-γ. Our results indicated that TLR2 signaling can direct PD-L2 expression on DCs, which binds to PD-1 mainly on CD4(+)T cells, to help inhibit T cells response in Schistosoma japonicum infection.
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spelling pubmed-38697112013-12-27 TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection Gao, Yanan Chen, Lin Hou, Min Chen, Yingying Ji, Minjun Wu, Haiwei Wu, Guanling PLoS One Research Article Toll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2(−/−)) mice compared with B6 mice. In Schistosoma japonicum chronic infection models, programmed death ligand 1 (PD-L1) and programmed death ligand 2 (PD-L2) expression as well as TLR2 expression gradually increased in B6 mice, while only PD-L2 expression significantly decreased in TLR2(−/−) mice. Meanwhile, Programmed Death 1(PD-1) expression on CD4(+)T cells was down-regulated in TLR2(−/−) mice after a large number of egg appeared. We also found that stimulation with schistosome antigens, especially SEA, could up-regulate PD-L2 expression on BMDCs in a TLR2-dependent manner in vitro. Schistosome antigens primed-BMDCs with impaired expression of TLR2 or PD-L2 could induce CD4(+)T cells to produce low level of IL-10 or high level of IFN-γ. Our results indicated that TLR2 signaling can direct PD-L2 expression on DCs, which binds to PD-1 mainly on CD4(+)T cells, to help inhibit T cells response in Schistosoma japonicum infection. Public Library of Science 2013-12-20 /pmc/articles/PMC3869711/ /pubmed/24376539 http://dx.doi.org/10.1371/journal.pone.0082480 Text en © 2013 Gao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gao, Yanan
Chen, Lin
Hou, Min
Chen, Yingying
Ji, Minjun
Wu, Haiwei
Wu, Guanling
TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title_full TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title_fullStr TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title_full_unstemmed TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title_short TLR2 Directing PD-L2 Expression Inhibit T Cells Response in Schistosoma japonicum Infection
title_sort tlr2 directing pd-l2 expression inhibit t cells response in schistosoma japonicum infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869711/
https://www.ncbi.nlm.nih.gov/pubmed/24376539
http://dx.doi.org/10.1371/journal.pone.0082480
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