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A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine

Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (seri...

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Autores principales: Philipp, Oliver, Hamann, Andrea, Servos, Jörg, Werner, Alexandra, Koch, Ina, Osiewacz, Heinz D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869774/
https://www.ncbi.nlm.nih.gov/pubmed/24376646
http://dx.doi.org/10.1371/journal.pone.0083109
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author Philipp, Oliver
Hamann, Andrea
Servos, Jörg
Werner, Alexandra
Koch, Ina
Osiewacz, Heinz D.
author_facet Philipp, Oliver
Hamann, Andrea
Servos, Jörg
Werner, Alexandra
Koch, Ina
Osiewacz, Heinz D.
author_sort Philipp, Oliver
collection PubMed
description Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control pathway that becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations.
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spelling pubmed-38697742013-12-27 A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine Philipp, Oliver Hamann, Andrea Servos, Jörg Werner, Alexandra Koch, Ina Osiewacz, Heinz D. PLoS One Research Article Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control pathway that becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations. Public Library of Science 2013-12-20 /pmc/articles/PMC3869774/ /pubmed/24376646 http://dx.doi.org/10.1371/journal.pone.0083109 Text en © 2013 Philipp et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Philipp, Oliver
Hamann, Andrea
Servos, Jörg
Werner, Alexandra
Koch, Ina
Osiewacz, Heinz D.
A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title_full A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title_fullStr A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title_full_unstemmed A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title_short A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
title_sort genome-wide longitudinal transcriptome analysis of the aging model podospora anserine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869774/
https://www.ncbi.nlm.nih.gov/pubmed/24376646
http://dx.doi.org/10.1371/journal.pone.0083109
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