Cargando…

Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens

Due to antimicrobial properties, nisin is one of the most commonly used and investigated bacteriocins for food preservation. Surprisingly, nisin has had limited use in animal feed as well as there are only few reports on its influence on microbial ecology of the gastrointestinal tract (GIT). The pre...

Descripción completa

Detalles Bibliográficos
Autores principales: Józefiak, Damian, Kierończyk, Bartosz, Juśkiewicz, Jerzy, Zduńczyk, Zenon, Rawski, Mateusz, Długosz, Jakub, Sip, Anna, Højberg, Ole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869907/
https://www.ncbi.nlm.nih.gov/pubmed/24376878
http://dx.doi.org/10.1371/journal.pone.0085347
_version_ 1782296634018758656
author Józefiak, Damian
Kierończyk, Bartosz
Juśkiewicz, Jerzy
Zduńczyk, Zenon
Rawski, Mateusz
Długosz, Jakub
Sip, Anna
Højberg, Ole
author_facet Józefiak, Damian
Kierończyk, Bartosz
Juśkiewicz, Jerzy
Zduńczyk, Zenon
Rawski, Mateusz
Długosz, Jakub
Sip, Anna
Højberg, Ole
author_sort Józefiak, Damian
collection PubMed
description Due to antimicrobial properties, nisin is one of the most commonly used and investigated bacteriocins for food preservation. Surprisingly, nisin has had limited use in animal feed as well as there are only few reports on its influence on microbial ecology of the gastrointestinal tract (GIT). The present study therefore aimed at investigating effects of dietary nisin on broiler chicken GIT microbial ecology and performance in comparison to salinomycin, the widely used ionophore coccidiostat. In total, 720 one-day-old male Ross 308 chicks were randomly distributed to six experimental groups. The positive control (PC) diet was supplemented with salinomycin (60 mg/kg). The nisin (NI) diets were supplemented with increasing levels (100, 300, 900 and 2700 IU nisin/g, respectively) of the bacteriocin. The negative control (NC) diet contained no additives. At slaughter (35 days of age), activity of specific bacterial enzymes (α- and β-glucosidases, α-galactosidases and β-glucuronidase) in crop, ileum and caeca were significantly higher (P<0.05) in the NC group, and nisin supplementation decreased the enzyme activities to levels observed for the PC group. A similar inhibitory influence on bacterial activity was reflected in the levels of short-chain fatty acids (SCFA) and putrefactive SCFA (PSCFA) in digesta from crop and ileum; no effect was observed in caeca. Counts of Bacteroides and Enterobacteriacae in ileum digesta were significantly (P<0.001) decreased by nisin and salinomycin, but no effects were observed on the counts of Clostridium perfringens, Lactobacillus/Enterococcus and total bacteria. Like salinomycin, nisin supplementation improved broiler growth performance in a dose-dependent manner; compared to the NC group, the body weight gain of the NI(900) and NI(2700) groups was improved by 4.7 and 8.7%, respectively. Our findings suggest that dietary nisin exerts a mode of action similar to salinomycin and could be considered as a dietary supplement for broiler chickens.
format Online
Article
Text
id pubmed-3869907
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38699072013-12-27 Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens Józefiak, Damian Kierończyk, Bartosz Juśkiewicz, Jerzy Zduńczyk, Zenon Rawski, Mateusz Długosz, Jakub Sip, Anna Højberg, Ole PLoS One Research Article Due to antimicrobial properties, nisin is one of the most commonly used and investigated bacteriocins for food preservation. Surprisingly, nisin has had limited use in animal feed as well as there are only few reports on its influence on microbial ecology of the gastrointestinal tract (GIT). The present study therefore aimed at investigating effects of dietary nisin on broiler chicken GIT microbial ecology and performance in comparison to salinomycin, the widely used ionophore coccidiostat. In total, 720 one-day-old male Ross 308 chicks were randomly distributed to six experimental groups. The positive control (PC) diet was supplemented with salinomycin (60 mg/kg). The nisin (NI) diets were supplemented with increasing levels (100, 300, 900 and 2700 IU nisin/g, respectively) of the bacteriocin. The negative control (NC) diet contained no additives. At slaughter (35 days of age), activity of specific bacterial enzymes (α- and β-glucosidases, α-galactosidases and β-glucuronidase) in crop, ileum and caeca were significantly higher (P<0.05) in the NC group, and nisin supplementation decreased the enzyme activities to levels observed for the PC group. A similar inhibitory influence on bacterial activity was reflected in the levels of short-chain fatty acids (SCFA) and putrefactive SCFA (PSCFA) in digesta from crop and ileum; no effect was observed in caeca. Counts of Bacteroides and Enterobacteriacae in ileum digesta were significantly (P<0.001) decreased by nisin and salinomycin, but no effects were observed on the counts of Clostridium perfringens, Lactobacillus/Enterococcus and total bacteria. Like salinomycin, nisin supplementation improved broiler growth performance in a dose-dependent manner; compared to the NC group, the body weight gain of the NI(900) and NI(2700) groups was improved by 4.7 and 8.7%, respectively. Our findings suggest that dietary nisin exerts a mode of action similar to salinomycin and could be considered as a dietary supplement for broiler chickens. Public Library of Science 2013-12-20 /pmc/articles/PMC3869907/ /pubmed/24376878 http://dx.doi.org/10.1371/journal.pone.0085347 Text en © 2013 Józefiak et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Józefiak, Damian
Kierończyk, Bartosz
Juśkiewicz, Jerzy
Zduńczyk, Zenon
Rawski, Mateusz
Długosz, Jakub
Sip, Anna
Højberg, Ole
Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title_full Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title_fullStr Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title_full_unstemmed Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title_short Dietary Nisin Modulates the Gastrointestinal Microbial Ecology and Enhances Growth Performance of the Broiler Chickens
title_sort dietary nisin modulates the gastrointestinal microbial ecology and enhances growth performance of the broiler chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869907/
https://www.ncbi.nlm.nih.gov/pubmed/24376878
http://dx.doi.org/10.1371/journal.pone.0085347
work_keys_str_mv AT jozefiakdamian dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT kieronczykbartosz dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT juskiewiczjerzy dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT zdunczykzenon dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT rawskimateusz dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT długoszjakub dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT sipanna dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens
AT højbergole dietarynisinmodulatesthegastrointestinalmicrobialecologyandenhancesgrowthperformanceofthebroilerchickens