Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis

We describe the expression of the extracellular domain of the human α1 nicotinic acetylcholine receptor (nAChR) in lepidopteran insect cells (i-α1-ECD) and its suitability for use in antigen-specific therapies for Myasthenia Gravis (MG). Compared to the previously expressed protein in P. pastoris (y...

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Autores principales: Niarchos, Athanasios, Zouridakis, Marios, Douris, Vassilis, Georgostathi, Assimina, Kalamida, Dimitra, Sotiriadis, Alexandros, Poulas, Konstantinos, Iatrou, Kostas, Tzartos, Socrates J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
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Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869910/
https://www.ncbi.nlm.nih.gov/pubmed/24376846
http://dx.doi.org/10.1371/journal.pone.0084791
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author Niarchos, Athanasios
Zouridakis, Marios
Douris, Vassilis
Georgostathi, Assimina
Kalamida, Dimitra
Sotiriadis, Alexandros
Poulas, Konstantinos
Iatrou, Kostas
Tzartos, Socrates J.
author_facet Niarchos, Athanasios
Zouridakis, Marios
Douris, Vassilis
Georgostathi, Assimina
Kalamida, Dimitra
Sotiriadis, Alexandros
Poulas, Konstantinos
Iatrou, Kostas
Tzartos, Socrates J.
author_sort Niarchos, Athanasios
collection PubMed
description We describe the expression of the extracellular domain of the human α1 nicotinic acetylcholine receptor (nAChR) in lepidopteran insect cells (i-α1-ECD) and its suitability for use in antigen-specific therapies for Myasthenia Gravis (MG). Compared to the previously expressed protein in P. pastoris (y-α1-ECD), i-α1-ECD had a 2-fold increased expression yield, bound anti-nAChR monoclonal antibodies and autoantibodies from MG patients two to several-fold more efficiently and resulted in a secondary structure closer to that of the crystal structure of mouse α1-ECD. Our results indicate that i-α1-ECD is an improved protein for use in antigen-specific MG therapeutic strategies.
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spelling pubmed-38699102013-12-27 Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis Niarchos, Athanasios Zouridakis, Marios Douris, Vassilis Georgostathi, Assimina Kalamida, Dimitra Sotiriadis, Alexandros Poulas, Konstantinos Iatrou, Kostas Tzartos, Socrates J. PLoS One Research Article We describe the expression of the extracellular domain of the human α1 nicotinic acetylcholine receptor (nAChR) in lepidopteran insect cells (i-α1-ECD) and its suitability for use in antigen-specific therapies for Myasthenia Gravis (MG). Compared to the previously expressed protein in P. pastoris (y-α1-ECD), i-α1-ECD had a 2-fold increased expression yield, bound anti-nAChR monoclonal antibodies and autoantibodies from MG patients two to several-fold more efficiently and resulted in a secondary structure closer to that of the crystal structure of mouse α1-ECD. Our results indicate that i-α1-ECD is an improved protein for use in antigen-specific MG therapeutic strategies. Public Library of Science 2013-12-20 /pmc/articles/PMC3869910/ /pubmed/24376846 http://dx.doi.org/10.1371/journal.pone.0084791 Text en © 2013 Niarchos et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Niarchos, Athanasios
Zouridakis, Marios
Douris, Vassilis
Georgostathi, Assimina
Kalamida, Dimitra
Sotiriadis, Alexandros
Poulas, Konstantinos
Iatrou, Kostas
Tzartos, Socrates J.
Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title_full Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title_fullStr Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title_full_unstemmed Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title_short Expression of a Highly Antigenic and Native-Like Folded Extracellular Domain of the Human α1 Subunit of Muscle Nicotinic Acetylcholine Receptor, Suitable for Use in Antigen Specific Therapies for Myasthenia Gravis
title_sort expression of a highly antigenic and native-like folded extracellular domain of the human α1 subunit of muscle nicotinic acetylcholine receptor, suitable for use in antigen specific therapies for myasthenia gravis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869910/
https://www.ncbi.nlm.nih.gov/pubmed/24376846
http://dx.doi.org/10.1371/journal.pone.0084791
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