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Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium

A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo(6+) to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybda...

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Autores principales: Ahmad, S. A., Shukor, M. Y., Shamaan, N. A., Mac Cormack, W. P., Syed, M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870105/
https://www.ncbi.nlm.nih.gov/pubmed/24381945
http://dx.doi.org/10.1155/2013/871941
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author Ahmad, S. A.
Shukor, M. Y.
Shamaan, N. A.
Mac Cormack, W. P.
Syed, M. A.
author_facet Ahmad, S. A.
Shukor, M. Y.
Shamaan, N. A.
Mac Cormack, W. P.
Syed, M. A.
author_sort Ahmad, S. A.
collection PubMed
description A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo(6+) to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries.
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spelling pubmed-38701052013-12-31 Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium Ahmad, S. A. Shukor, M. Y. Shamaan, N. A. Mac Cormack, W. P. Syed, M. A. Biomed Res Int Research Article A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo(6+) to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries. Hindawi Publishing Corporation 2013 2013-12-05 /pmc/articles/PMC3870105/ /pubmed/24381945 http://dx.doi.org/10.1155/2013/871941 Text en Copyright © 2013 S. A. Ahmad et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ahmad, S. A.
Shukor, M. Y.
Shamaan, N. A.
Mac Cormack, W. P.
Syed, M. A.
Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title_full Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title_fullStr Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title_full_unstemmed Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title_short Molybdate Reduction to Molybdenum Blue by an Antarctic Bacterium
title_sort molybdate reduction to molybdenum blue by an antarctic bacterium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870105/
https://www.ncbi.nlm.nih.gov/pubmed/24381945
http://dx.doi.org/10.1155/2013/871941
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