Cargando…

Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis

Mesenchymal stromal cells (MSCs) are currently being investigated in numerous clinical trials of tissue repair and various immunological disorders based on their ability to secrete trophic factors and to modulate inflammatory responses. MSCs have been shown to migrate to sites of injury and inflamma...

Descripción completa

Detalles Bibliográficos
Autores principales: Marquez-Curtis, Leah A., Janowska-Wieczorek, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870125/
https://www.ncbi.nlm.nih.gov/pubmed/24381939
http://dx.doi.org/10.1155/2013/561098
_version_ 1782296665919586304
author Marquez-Curtis, Leah A.
Janowska-Wieczorek, Anna
author_facet Marquez-Curtis, Leah A.
Janowska-Wieczorek, Anna
author_sort Marquez-Curtis, Leah A.
collection PubMed
description Mesenchymal stromal cells (MSCs) are currently being investigated in numerous clinical trials of tissue repair and various immunological disorders based on their ability to secrete trophic factors and to modulate inflammatory responses. MSCs have been shown to migrate to sites of injury and inflammation in response to soluble mediators including the chemokine stromal cell-derived factor-(SDF-)1, but during in vitro culture expansion MSCs lose surface expression of key homing receptors particularly of the SDF-1 receptor, CXCR4. Here we review studies on enhancement of SDF-1-directed migration of MSCs with the premise that their improved recruitment could translate to therapeutic benefits. We describe our studies on approaches to increase the CXCR4 expression in in vitro-expanded cord blood-derived MSCs, namely, transfection, using the commercial liposomal reagent IBAfect, chemical treatment with the histone deacetylase inhibitor valproic acid, and exposure to recombinant complement component C1q. These methodologies will be presented in the context of other cell targeting and delivery strategies that exploit pathways involved in MSC migration. Taken together, these findings indicate that MSCs can be manipulated in vitro to enhance their in vivo recruitment and efficacy for tissue repair.
format Online
Article
Text
id pubmed-3870125
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38701252013-12-31 Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis Marquez-Curtis, Leah A. Janowska-Wieczorek, Anna Biomed Res Int Review Article Mesenchymal stromal cells (MSCs) are currently being investigated in numerous clinical trials of tissue repair and various immunological disorders based on their ability to secrete trophic factors and to modulate inflammatory responses. MSCs have been shown to migrate to sites of injury and inflammation in response to soluble mediators including the chemokine stromal cell-derived factor-(SDF-)1, but during in vitro culture expansion MSCs lose surface expression of key homing receptors particularly of the SDF-1 receptor, CXCR4. Here we review studies on enhancement of SDF-1-directed migration of MSCs with the premise that their improved recruitment could translate to therapeutic benefits. We describe our studies on approaches to increase the CXCR4 expression in in vitro-expanded cord blood-derived MSCs, namely, transfection, using the commercial liposomal reagent IBAfect, chemical treatment with the histone deacetylase inhibitor valproic acid, and exposure to recombinant complement component C1q. These methodologies will be presented in the context of other cell targeting and delivery strategies that exploit pathways involved in MSC migration. Taken together, these findings indicate that MSCs can be manipulated in vitro to enhance their in vivo recruitment and efficacy for tissue repair. Hindawi Publishing Corporation 2013 2013-12-05 /pmc/articles/PMC3870125/ /pubmed/24381939 http://dx.doi.org/10.1155/2013/561098 Text en Copyright © 2013 L. A. Marquez-Curtis and A. Janowska-Wieczorek. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Marquez-Curtis, Leah A.
Janowska-Wieczorek, Anna
Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title_full Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title_fullStr Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title_full_unstemmed Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title_short Enhancing the Migration Ability of Mesenchymal Stromal Cells by Targeting the SDF-1/CXCR4 Axis
title_sort enhancing the migration ability of mesenchymal stromal cells by targeting the sdf-1/cxcr4 axis
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870125/
https://www.ncbi.nlm.nih.gov/pubmed/24381939
http://dx.doi.org/10.1155/2013/561098
work_keys_str_mv AT marquezcurtisleaha enhancingthemigrationabilityofmesenchymalstromalcellsbytargetingthesdf1cxcr4axis
AT janowskawieczorekanna enhancingthemigrationabilityofmesenchymalstromalcellsbytargetingthesdf1cxcr4axis