Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains

In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(−6) per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element...

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Autores principales: Goh, Shan, Hussain, Haitham, Chang, Barbara J., Emmett, Warren, Riley, Thomas V., Mullany, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870246/
https://www.ncbi.nlm.nih.gov/pubmed/24255122
http://dx.doi.org/10.1128/mBio.00840-13
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author Goh, Shan
Hussain, Haitham
Chang, Barbara J.
Emmett, Warren
Riley, Thomas V.
Mullany, Peter
author_facet Goh, Shan
Hussain, Haitham
Chang, Barbara J.
Emmett, Warren
Riley, Thomas V.
Mullany, Peter
author_sort Goh, Shan
collection PubMed
description In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(−6) per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008 bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating.
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spelling pubmed-38702462013-12-26 Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains Goh, Shan Hussain, Haitham Chang, Barbara J. Emmett, Warren Riley, Thomas V. Mullany, Peter mBio Research Article In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(−6) per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008 bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating. American Society of Microbiology 2013-11-19 /pmc/articles/PMC3870246/ /pubmed/24255122 http://dx.doi.org/10.1128/mBio.00840-13 Text en Copyright © 2013 Goh et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Goh, Shan
Hussain, Haitham
Chang, Barbara J.
Emmett, Warren
Riley, Thomas V.
Mullany, Peter
Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title_full Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title_fullStr Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title_full_unstemmed Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title_short Phage ϕC2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains
title_sort phage ϕc2 mediates transduction of tn6215, encoding erythromycin resistance, between clostridium difficile strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870246/
https://www.ncbi.nlm.nih.gov/pubmed/24255122
http://dx.doi.org/10.1128/mBio.00840-13
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