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c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation

BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the en...

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Autores principales: Zhang, Lei, Tang, Jiangjing, Haines, Christopher J, Feng, Huai, Lai, Liangxue, Teng, Xiaoming, Han, Yibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871025/
https://www.ncbi.nlm.nih.gov/pubmed/24161026
http://dx.doi.org/10.1186/1471-213X-13-38
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author Zhang, Lei
Tang, Jiangjing
Haines, Christopher J
Feng, Huai
Lai, Liangxue
Teng, Xiaoming
Han, Yibing
author_facet Zhang, Lei
Tang, Jiangjing
Haines, Christopher J
Feng, Huai
Lai, Liangxue
Teng, Xiaoming
Han, Yibing
author_sort Zhang, Lei
collection PubMed
description BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the entire spermatogenesis process is still unclear. This study aims to reveal and compare c-kit expression profiles in the SSCs before and after the anticipated differentiation, as well as to examine its relationship with retinoic acid (RA) stimulation. RESULTS: We have found that there are more than 4 transcripts of c-kit expressed in the cell lines and in the testes. The transcripts can be divided into short and long categories. The long transcripts include the full-length canonical c-kit transcript and the 3′ end short transcript. Short transcripts include the 3.4 kb short transcript and several truncated transcripts (1.9-3.2 kb). In addition, the 3.4 kb transcript (starting from intron 9 and covering exons 10 ~ 21) is discovered to be specifically expressed in the spermatogonia. The extracellular domain of Kit is obtained in the spermatogonia stage, but the intracellular domain (50 kDa) is constantly expressed in both SSCs and spermatogonia. The c-kit expression profiles in the testis and the spermatogonial stem cell lines vary after RA stimulation. The wave-like changes of the quantitative expression pattern of c-kit (increase initially and decrease afterwards) during the induction process are similar to that of the in vivo male germ cell development process. CONCLUSIONS: There are dynamic transcription and translation changes of c-kit before and after SSCs’ anticipated differentiation and most importantly, RA is a significant upstream regulatory factor for c-kit expression.
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spelling pubmed-38710252013-12-25 c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation Zhang, Lei Tang, Jiangjing Haines, Christopher J Feng, Huai Lai, Liangxue Teng, Xiaoming Han, Yibing BMC Dev Biol Research Article BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the entire spermatogenesis process is still unclear. This study aims to reveal and compare c-kit expression profiles in the SSCs before and after the anticipated differentiation, as well as to examine its relationship with retinoic acid (RA) stimulation. RESULTS: We have found that there are more than 4 transcripts of c-kit expressed in the cell lines and in the testes. The transcripts can be divided into short and long categories. The long transcripts include the full-length canonical c-kit transcript and the 3′ end short transcript. Short transcripts include the 3.4 kb short transcript and several truncated transcripts (1.9-3.2 kb). In addition, the 3.4 kb transcript (starting from intron 9 and covering exons 10 ~ 21) is discovered to be specifically expressed in the spermatogonia. The extracellular domain of Kit is obtained in the spermatogonia stage, but the intracellular domain (50 kDa) is constantly expressed in both SSCs and spermatogonia. The c-kit expression profiles in the testis and the spermatogonial stem cell lines vary after RA stimulation. The wave-like changes of the quantitative expression pattern of c-kit (increase initially and decrease afterwards) during the induction process are similar to that of the in vivo male germ cell development process. CONCLUSIONS: There are dynamic transcription and translation changes of c-kit before and after SSCs’ anticipated differentiation and most importantly, RA is a significant upstream regulatory factor for c-kit expression. BioMed Central 2013-10-27 /pmc/articles/PMC3871025/ /pubmed/24161026 http://dx.doi.org/10.1186/1471-213X-13-38 Text en Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lei
Tang, Jiangjing
Haines, Christopher J
Feng, Huai
Lai, Liangxue
Teng, Xiaoming
Han, Yibing
c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title_full c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title_fullStr c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title_full_unstemmed c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title_short c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
title_sort c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871025/
https://www.ncbi.nlm.nih.gov/pubmed/24161026
http://dx.doi.org/10.1186/1471-213X-13-38
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