Cargando…
c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the en...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871025/ https://www.ncbi.nlm.nih.gov/pubmed/24161026 http://dx.doi.org/10.1186/1471-213X-13-38 |
_version_ | 1782296761385091072 |
---|---|
author | Zhang, Lei Tang, Jiangjing Haines, Christopher J Feng, Huai Lai, Liangxue Teng, Xiaoming Han, Yibing |
author_facet | Zhang, Lei Tang, Jiangjing Haines, Christopher J Feng, Huai Lai, Liangxue Teng, Xiaoming Han, Yibing |
author_sort | Zhang, Lei |
collection | PubMed |
description | BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the entire spermatogenesis process is still unclear. This study aims to reveal and compare c-kit expression profiles in the SSCs before and after the anticipated differentiation, as well as to examine its relationship with retinoic acid (RA) stimulation. RESULTS: We have found that there are more than 4 transcripts of c-kit expressed in the cell lines and in the testes. The transcripts can be divided into short and long categories. The long transcripts include the full-length canonical c-kit transcript and the 3′ end short transcript. Short transcripts include the 3.4 kb short transcript and several truncated transcripts (1.9-3.2 kb). In addition, the 3.4 kb transcript (starting from intron 9 and covering exons 10 ~ 21) is discovered to be specifically expressed in the spermatogonia. The extracellular domain of Kit is obtained in the spermatogonia stage, but the intracellular domain (50 kDa) is constantly expressed in both SSCs and spermatogonia. The c-kit expression profiles in the testis and the spermatogonial stem cell lines vary after RA stimulation. The wave-like changes of the quantitative expression pattern of c-kit (increase initially and decrease afterwards) during the induction process are similar to that of the in vivo male germ cell development process. CONCLUSIONS: There are dynamic transcription and translation changes of c-kit before and after SSCs’ anticipated differentiation and most importantly, RA is a significant upstream regulatory factor for c-kit expression. |
format | Online Article Text |
id | pubmed-3871025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38710252013-12-25 c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation Zhang, Lei Tang, Jiangjing Haines, Christopher J Feng, Huai Lai, Liangxue Teng, Xiaoming Han, Yibing BMC Dev Biol Research Article BACKGROUND: It has been proven that c-kit is crucial for proliferation, migration, survival and maturation of spermatogenic cells. A periodic expression of c-kit is observed from primordial germ cells (PGCs) to spermatogenetic stem cells (SSCs), However, the expression profile of c-kit during the entire spermatogenesis process is still unclear. This study aims to reveal and compare c-kit expression profiles in the SSCs before and after the anticipated differentiation, as well as to examine its relationship with retinoic acid (RA) stimulation. RESULTS: We have found that there are more than 4 transcripts of c-kit expressed in the cell lines and in the testes. The transcripts can be divided into short and long categories. The long transcripts include the full-length canonical c-kit transcript and the 3′ end short transcript. Short transcripts include the 3.4 kb short transcript and several truncated transcripts (1.9-3.2 kb). In addition, the 3.4 kb transcript (starting from intron 9 and covering exons 10 ~ 21) is discovered to be specifically expressed in the spermatogonia. The extracellular domain of Kit is obtained in the spermatogonia stage, but the intracellular domain (50 kDa) is constantly expressed in both SSCs and spermatogonia. The c-kit expression profiles in the testis and the spermatogonial stem cell lines vary after RA stimulation. The wave-like changes of the quantitative expression pattern of c-kit (increase initially and decrease afterwards) during the induction process are similar to that of the in vivo male germ cell development process. CONCLUSIONS: There are dynamic transcription and translation changes of c-kit before and after SSCs’ anticipated differentiation and most importantly, RA is a significant upstream regulatory factor for c-kit expression. BioMed Central 2013-10-27 /pmc/articles/PMC3871025/ /pubmed/24161026 http://dx.doi.org/10.1186/1471-213X-13-38 Text en Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Lei Tang, Jiangjing Haines, Christopher J Feng, Huai Lai, Liangxue Teng, Xiaoming Han, Yibing c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title_full | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title_fullStr | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title_full_unstemmed | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title_short | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
title_sort | c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871025/ https://www.ncbi.nlm.nih.gov/pubmed/24161026 http://dx.doi.org/10.1186/1471-213X-13-38 |
work_keys_str_mv | AT zhanglei ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT tangjiangjing ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT haineschristopherj ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT fenghuai ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT lailiangxue ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT tengxiaoming ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation AT hanyibing ckitexpressionprofileandregulatoryfactorsduringspermatogonialstemcelldifferentiation |