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Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus
The discovery of quorum sensing in Proteobacteria and its function in regulating virulence determinants makes it an attractive alternative towards attenuation of bacterial pathogens. In this study, crude extracts of Phyllanthus amarus Schumach. & Thonn, a traditional Chinese herb, were screened...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871092/ https://www.ncbi.nlm.nih.gov/pubmed/24169540 http://dx.doi.org/10.3390/s131114558 |
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author | Priya, Kumutha Yin, Wai-Fong Chan, Kok-Gan |
author_facet | Priya, Kumutha Yin, Wai-Fong Chan, Kok-Gan |
author_sort | Priya, Kumutha |
collection | PubMed |
description | The discovery of quorum sensing in Proteobacteria and its function in regulating virulence determinants makes it an attractive alternative towards attenuation of bacterial pathogens. In this study, crude extracts of Phyllanthus amarus Schumach. & Thonn, a traditional Chinese herb, were screened for their anti-quorum sensing properties through a series of bioassays. Only the methanolic extract of P. amarus exhibited anti-quorum sensing activity, whereby it interrupted the ability of Chromobacterium violaceum CVO26 to response towards exogenously supplied N-hexanoylhomoserine lactone and the extract reduced bioluminescence in E. coli [pSB401] and E. coli [pSB1075]. In addition to this, methanolic extract of P. amarus significantly inhibited selected quorum sensing-regulated virulence determinants of Pseudomonas aeruginosa PA01. Increasing concentrations of the methanolic extracts of P. amarus reduced swarming motility, pyocyanin production and P. aeruginosa PA01 lecA∷lux expression. Our data suggest that P. amarus could be useful for attenuating pathogens and hence, more local traditional herbs should be screened for its anti-quorum sensing properties as their active compounds may serve as promising anti-pathogenic drugs. |
format | Online Article Text |
id | pubmed-3871092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-38710922013-12-26 Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus Priya, Kumutha Yin, Wai-Fong Chan, Kok-Gan Sensors (Basel) Article The discovery of quorum sensing in Proteobacteria and its function in regulating virulence determinants makes it an attractive alternative towards attenuation of bacterial pathogens. In this study, crude extracts of Phyllanthus amarus Schumach. & Thonn, a traditional Chinese herb, were screened for their anti-quorum sensing properties through a series of bioassays. Only the methanolic extract of P. amarus exhibited anti-quorum sensing activity, whereby it interrupted the ability of Chromobacterium violaceum CVO26 to response towards exogenously supplied N-hexanoylhomoserine lactone and the extract reduced bioluminescence in E. coli [pSB401] and E. coli [pSB1075]. In addition to this, methanolic extract of P. amarus significantly inhibited selected quorum sensing-regulated virulence determinants of Pseudomonas aeruginosa PA01. Increasing concentrations of the methanolic extracts of P. amarus reduced swarming motility, pyocyanin production and P. aeruginosa PA01 lecA∷lux expression. Our data suggest that P. amarus could be useful for attenuating pathogens and hence, more local traditional herbs should be screened for its anti-quorum sensing properties as their active compounds may serve as promising anti-pathogenic drugs. Molecular Diversity Preservation International (MDPI) 2013-10-28 /pmc/articles/PMC3871092/ /pubmed/24169540 http://dx.doi.org/10.3390/s131114558 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Priya, Kumutha Yin, Wai-Fong Chan, Kok-Gan Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title | Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title_full | Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title_fullStr | Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title_full_unstemmed | Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title_short | Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus |
title_sort | anti-quorum sensing activity of the traditional chinese herb, phyllanthus amarus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871092/ https://www.ncbi.nlm.nih.gov/pubmed/24169540 http://dx.doi.org/10.3390/s131114558 |
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