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Autophagy and metacaspase determine the mode of cell death in plants

Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, ac...

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Autores principales: Minina, Elena A., Filonova, Lada H., Fukada, Kazutake, Savenkov, Eugene I., Gogvadze, Vladimir, Clapham, David, Sanchez-Vera, Victoria, Suarez, Maria F., Zhivotovsky, Boris, Daniel, Geoffrey, Smertenko, Andrei, Bozhkov, Peter V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871426/
https://www.ncbi.nlm.nih.gov/pubmed/24344187
http://dx.doi.org/10.1083/jcb.201307082
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author Minina, Elena A.
Filonova, Lada H.
Fukada, Kazutake
Savenkov, Eugene I.
Gogvadze, Vladimir
Clapham, David
Sanchez-Vera, Victoria
Suarez, Maria F.
Zhivotovsky, Boris
Daniel, Geoffrey
Smertenko, Andrei
Bozhkov, Peter V.
author_facet Minina, Elena A.
Filonova, Lada H.
Fukada, Kazutake
Savenkov, Eugene I.
Gogvadze, Vladimir
Clapham, David
Sanchez-Vera, Victoria
Suarez, Maria F.
Zhivotovsky, Boris
Daniel, Geoffrey
Smertenko, Andrei
Bozhkov, Peter V.
author_sort Minina, Elena A.
collection PubMed
description Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is characterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains obscure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase–autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase-dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis.
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spelling pubmed-38714262014-06-23 Autophagy and metacaspase determine the mode of cell death in plants Minina, Elena A. Filonova, Lada H. Fukada, Kazutake Savenkov, Eugene I. Gogvadze, Vladimir Clapham, David Sanchez-Vera, Victoria Suarez, Maria F. Zhivotovsky, Boris Daniel, Geoffrey Smertenko, Andrei Bozhkov, Peter V. J Cell Biol Research Articles Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is characterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains obscure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase–autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase-dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis. The Rockefeller University Press 2013-12-23 /pmc/articles/PMC3871426/ /pubmed/24344187 http://dx.doi.org/10.1083/jcb.201307082 Text en © 2013 Minina et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Minina, Elena A.
Filonova, Lada H.
Fukada, Kazutake
Savenkov, Eugene I.
Gogvadze, Vladimir
Clapham, David
Sanchez-Vera, Victoria
Suarez, Maria F.
Zhivotovsky, Boris
Daniel, Geoffrey
Smertenko, Andrei
Bozhkov, Peter V.
Autophagy and metacaspase determine the mode of cell death in plants
title Autophagy and metacaspase determine the mode of cell death in plants
title_full Autophagy and metacaspase determine the mode of cell death in plants
title_fullStr Autophagy and metacaspase determine the mode of cell death in plants
title_full_unstemmed Autophagy and metacaspase determine the mode of cell death in plants
title_short Autophagy and metacaspase determine the mode of cell death in plants
title_sort autophagy and metacaspase determine the mode of cell death in plants
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871426/
https://www.ncbi.nlm.nih.gov/pubmed/24344187
http://dx.doi.org/10.1083/jcb.201307082
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