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Autophagy and metacaspase determine the mode of cell death in plants
Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, ac...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871426/ https://www.ncbi.nlm.nih.gov/pubmed/24344187 http://dx.doi.org/10.1083/jcb.201307082 |
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author | Minina, Elena A. Filonova, Lada H. Fukada, Kazutake Savenkov, Eugene I. Gogvadze, Vladimir Clapham, David Sanchez-Vera, Victoria Suarez, Maria F. Zhivotovsky, Boris Daniel, Geoffrey Smertenko, Andrei Bozhkov, Peter V. |
author_facet | Minina, Elena A. Filonova, Lada H. Fukada, Kazutake Savenkov, Eugene I. Gogvadze, Vladimir Clapham, David Sanchez-Vera, Victoria Suarez, Maria F. Zhivotovsky, Boris Daniel, Geoffrey Smertenko, Andrei Bozhkov, Peter V. |
author_sort | Minina, Elena A. |
collection | PubMed |
description | Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is characterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains obscure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase–autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase-dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis. |
format | Online Article Text |
id | pubmed-3871426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38714262014-06-23 Autophagy and metacaspase determine the mode of cell death in plants Minina, Elena A. Filonova, Lada H. Fukada, Kazutake Savenkov, Eugene I. Gogvadze, Vladimir Clapham, David Sanchez-Vera, Victoria Suarez, Maria F. Zhivotovsky, Boris Daniel, Geoffrey Smertenko, Andrei Bozhkov, Peter V. J Cell Biol Research Articles Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell-autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is characterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains obscure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase–autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase-dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis. The Rockefeller University Press 2013-12-23 /pmc/articles/PMC3871426/ /pubmed/24344187 http://dx.doi.org/10.1083/jcb.201307082 Text en © 2013 Minina et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Minina, Elena A. Filonova, Lada H. Fukada, Kazutake Savenkov, Eugene I. Gogvadze, Vladimir Clapham, David Sanchez-Vera, Victoria Suarez, Maria F. Zhivotovsky, Boris Daniel, Geoffrey Smertenko, Andrei Bozhkov, Peter V. Autophagy and metacaspase determine the mode of cell death in plants |
title | Autophagy and metacaspase determine the mode of cell death in plants |
title_full | Autophagy and metacaspase determine the mode of cell death in plants |
title_fullStr | Autophagy and metacaspase determine the mode of cell death in plants |
title_full_unstemmed | Autophagy and metacaspase determine the mode of cell death in plants |
title_short | Autophagy and metacaspase determine the mode of cell death in plants |
title_sort | autophagy and metacaspase determine the mode of cell death in plants |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871426/ https://www.ncbi.nlm.nih.gov/pubmed/24344187 http://dx.doi.org/10.1083/jcb.201307082 |
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