Cargando…

Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts

Skin complications were recently reported after carbon-ion (C-ion) radiation therapy. Oxidative stress is considered an important pathway in the appearance of late skin reactions. We evaluated oxidative stress in normal human skin fibroblasts after carbon-ion vs. X-ray irradiation. Survival curves a...

Descripción completa

Detalles Bibliográficos
Autores principales: Laurent, Carine, Leduc, Alexandre, Pottier, Ivannah, Prévost, Virginie, Sichel, François, Lefaix, Jean-Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871598/
https://www.ncbi.nlm.nih.gov/pubmed/24376870
http://dx.doi.org/10.1371/journal.pone.0085158
_version_ 1782296843947868160
author Laurent, Carine
Leduc, Alexandre
Pottier, Ivannah
Prévost, Virginie
Sichel, François
Lefaix, Jean-Louis
author_facet Laurent, Carine
Leduc, Alexandre
Pottier, Ivannah
Prévost, Virginie
Sichel, François
Lefaix, Jean-Louis
author_sort Laurent, Carine
collection PubMed
description Skin complications were recently reported after carbon-ion (C-ion) radiation therapy. Oxidative stress is considered an important pathway in the appearance of late skin reactions. We evaluated oxidative stress in normal human skin fibroblasts after carbon-ion vs. X-ray irradiation. Survival curves and radiobiological parameters were calculated. DNA damage was quantified, as were lipid peroxidation (LPO), protein carbonylation and antioxidant enzyme activities. Reduced and oxidized glutathione ratios (GSH/GSSG) were determined. Proinflammatory cytokine secretion in culture supernatants was evaluated. The relative biological effectiveness (RBE) of C-ions vs. X-rays was 4.8 at D(0) (irradiation dose corresponding to a surviving fraction of 37%). Surviving fraction at 2 Gy (SF2) was 71.8% and 7.6% for X-rays and C-ions, respectively. Compared with X-rays, immediate DNA damage was increased less after C-ions, but a late increase was observed at D(10%) (irradiation dose corresponding to a surviving fraction of 10%). LPO products and protein carbonyls were only increased 24 hours after C-ions. After X-rays, superoxide dismutase (SOD) activity was strongly increased immediately and on day 14 at D(0%) (irradiation dose corresponding to a surviving fraction of around 0%), catalase activity was unchanged and glutathione peroxidase (GPx) activity was increased only on day 14. These activities were decreased after C-ions compared with X-rays. GSH/GSSG was unchanged after X-rays but was decreased immediately after C-ion irradiation before an increase from day 7. Secretion of IL-6 was increased at late times after X-ray irradiation. After C-ion irradiation, IL-6 concentration was increased on day 7 but was lower compared with X-rays at later times. C-ion effects on normal human skin fibroblasts seemed to be harmful in comparison with X-rays as they produce late DNA damage, LPO products and protein carbonyls, and as they decrease antioxidant defences. Mechanisms leading to this discrepancy between the two types of radiation should be investigated.
format Online
Article
Text
id pubmed-3871598
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38715982013-12-27 Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts Laurent, Carine Leduc, Alexandre Pottier, Ivannah Prévost, Virginie Sichel, François Lefaix, Jean-Louis PLoS One Research Article Skin complications were recently reported after carbon-ion (C-ion) radiation therapy. Oxidative stress is considered an important pathway in the appearance of late skin reactions. We evaluated oxidative stress in normal human skin fibroblasts after carbon-ion vs. X-ray irradiation. Survival curves and radiobiological parameters were calculated. DNA damage was quantified, as were lipid peroxidation (LPO), protein carbonylation and antioxidant enzyme activities. Reduced and oxidized glutathione ratios (GSH/GSSG) were determined. Proinflammatory cytokine secretion in culture supernatants was evaluated. The relative biological effectiveness (RBE) of C-ions vs. X-rays was 4.8 at D(0) (irradiation dose corresponding to a surviving fraction of 37%). Surviving fraction at 2 Gy (SF2) was 71.8% and 7.6% for X-rays and C-ions, respectively. Compared with X-rays, immediate DNA damage was increased less after C-ions, but a late increase was observed at D(10%) (irradiation dose corresponding to a surviving fraction of 10%). LPO products and protein carbonyls were only increased 24 hours after C-ions. After X-rays, superoxide dismutase (SOD) activity was strongly increased immediately and on day 14 at D(0%) (irradiation dose corresponding to a surviving fraction of around 0%), catalase activity was unchanged and glutathione peroxidase (GPx) activity was increased only on day 14. These activities were decreased after C-ions compared with X-rays. GSH/GSSG was unchanged after X-rays but was decreased immediately after C-ion irradiation before an increase from day 7. Secretion of IL-6 was increased at late times after X-ray irradiation. After C-ion irradiation, IL-6 concentration was increased on day 7 but was lower compared with X-rays at later times. C-ion effects on normal human skin fibroblasts seemed to be harmful in comparison with X-rays as they produce late DNA damage, LPO products and protein carbonyls, and as they decrease antioxidant defences. Mechanisms leading to this discrepancy between the two types of radiation should be investigated. Public Library of Science 2013-12-23 /pmc/articles/PMC3871598/ /pubmed/24376870 http://dx.doi.org/10.1371/journal.pone.0085158 Text en © 2013 Laurent et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Laurent, Carine
Leduc, Alexandre
Pottier, Ivannah
Prévost, Virginie
Sichel, François
Lefaix, Jean-Louis
Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title_full Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title_fullStr Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title_full_unstemmed Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title_short Dramatic Increase in Oxidative Stress in Carbon-Irradiated Normal Human Skin Fibroblasts
title_sort dramatic increase in oxidative stress in carbon-irradiated normal human skin fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871598/
https://www.ncbi.nlm.nih.gov/pubmed/24376870
http://dx.doi.org/10.1371/journal.pone.0085158
work_keys_str_mv AT laurentcarine dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts
AT leducalexandre dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts
AT pottierivannah dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts
AT prevostvirginie dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts
AT sichelfrancois dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts
AT lefaixjeanlouis dramaticincreaseinoxidativestressincarbonirradiatednormalhumanskinfibroblasts