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Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans
The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871683/ https://www.ncbi.nlm.nih.gov/pubmed/24376796 http://dx.doi.org/10.1371/journal.pone.0084201 |
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author | Lee, Hyo-Seol Lee, Jongeun Kim, Seong-Oh Song, Je-Seon Lee, Jae-Ho Lee, Syng-Ill Jung, Han-Sung Choi, Byung-Jai |
author_facet | Lee, Hyo-Seol Lee, Jongeun Kim, Seong-Oh Song, Je-Seon Lee, Jae-Ho Lee, Syng-Ill Jung, Han-Sung Choi, Byung-Jai |
author_sort | Lee, Hyo-Seol |
collection | PubMed |
description | The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL. |
format | Online Article Text |
id | pubmed-3871683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38716832013-12-27 Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans Lee, Hyo-Seol Lee, Jongeun Kim, Seong-Oh Song, Je-Seon Lee, Jae-Ho Lee, Syng-Ill Jung, Han-Sung Choi, Byung-Jai PLoS One Research Article The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL. Public Library of Science 2013-12-23 /pmc/articles/PMC3871683/ /pubmed/24376796 http://dx.doi.org/10.1371/journal.pone.0084201 Text en © 2013 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lee, Hyo-Seol Lee, Jongeun Kim, Seong-Oh Song, Je-Seon Lee, Jae-Ho Lee, Syng-Ill Jung, Han-Sung Choi, Byung-Jai Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title | Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title_full | Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title_fullStr | Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title_full_unstemmed | Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title_short | Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans |
title_sort | comparative gene-expression analysis of the dental follicle and periodontal ligament in humans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871683/ https://www.ncbi.nlm.nih.gov/pubmed/24376796 http://dx.doi.org/10.1371/journal.pone.0084201 |
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