Cargando…
The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands
The synthesized cobalt(II) complex, CoPNBDENA and the binding of this complex with sheep genomic DNA were investigated by UV–Visible absorption and viscosity techniques. Also the interaction of sheep genomic DNA with the complex was studied using the agarose gel electrophoresis method. The results i...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871802/ |
_version_ | 1782296879819653120 |
---|---|
author | Arslantas, Ali Devrim, A. Kadir Necefoglu, Hacali |
author_facet | Arslantas, Ali Devrim, A. Kadir Necefoglu, Hacali |
author_sort | Arslantas, Ali |
collection | PubMed |
description | The synthesized cobalt(II) complex, CoPNBDENA and the binding of this complex with sheep genomic DNA were investigated by UV–Visible absorption and viscosity techniques. Also the interaction of sheep genomic DNA with the complex was studied using the agarose gel electrophoresis method. The results indicated that the complex interacted with DNA. The nature of the binding seemed to be mainly an electrostatic interaction between DNA and the cobalt(II) complex. Other binding modes such as hydrogen bonds may also exist in this system. In this study, after the interaction of DNA–CoPNBDENA, it was observed that the migration of the DNA band became slow as the amount of cobalt(II) complex was increased. This clearly demonstrates that the CoPNBDENA complex neutralizes the negative charges of DNA. |
format | Online Article Text |
id | pubmed-3871802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-38718022013-12-26 The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands Arslantas, Ali Devrim, A. Kadir Necefoglu, Hacali Int J Mol Sci Full Research Paper The synthesized cobalt(II) complex, CoPNBDENA and the binding of this complex with sheep genomic DNA were investigated by UV–Visible absorption and viscosity techniques. Also the interaction of sheep genomic DNA with the complex was studied using the agarose gel electrophoresis method. The results indicated that the complex interacted with DNA. The nature of the binding seemed to be mainly an electrostatic interaction between DNA and the cobalt(II) complex. Other binding modes such as hydrogen bonds may also exist in this system. In this study, after the interaction of DNA–CoPNBDENA, it was observed that the migration of the DNA band became slow as the amount of cobalt(II) complex was increased. This clearly demonstrates that the CoPNBDENA complex neutralizes the negative charges of DNA. Molecular Diversity Preservation International (MDPI) 2007-12-13 /pmc/articles/PMC3871802/ Text en © 2007 by MDPI Reproduction is permitted for noncommercial purposes. |
spellingShingle | Full Research Paper Arslantas, Ali Devrim, A. Kadir Necefoglu, Hacali The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title | The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title_full | The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title_fullStr | The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title_full_unstemmed | The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title_short | The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N′-Diethylnicotinamide Ligands |
title_sort | interaction of sheep genomic dna with a cobalt(ii) complex containing p-nitrobenzoate and n,n′-diethylnicotinamide ligands |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871802/ |
work_keys_str_mv | AT arslantasali theinteractionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands AT devrimakadir theinteractionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands AT necefogluhacali theinteractionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands AT arslantasali interactionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands AT devrimakadir interactionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands AT necefogluhacali interactionofsheepgenomicdnawithacobaltiicomplexcontainingpnitrobenzoateandnndiethylnicotinamideligands |