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Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin

Leguminous biological nitrogen fixation (BNF) is very sensitive to environmental fluctuations. It is still contentious how BNF is regulated under stress conditions. The local or systemic control of BNF and the role played by reactive oxygen species (ROS) in such regulation have still not been elucid...

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Autores principales: Marino, Daniel, Damiani, Isabelle, Gucciardo, Sébastien, Mijangos, Iker, Pauly, Nicolas, Puppo, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871818/
https://www.ncbi.nlm.nih.gov/pubmed/24151304
http://dx.doi.org/10.1093/jxb/ert334
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author Marino, Daniel
Damiani, Isabelle
Gucciardo, Sébastien
Mijangos, Iker
Pauly, Nicolas
Puppo, Alain
author_facet Marino, Daniel
Damiani, Isabelle
Gucciardo, Sébastien
Mijangos, Iker
Pauly, Nicolas
Puppo, Alain
author_sort Marino, Daniel
collection PubMed
description Leguminous biological nitrogen fixation (BNF) is very sensitive to environmental fluctuations. It is still contentious how BNF is regulated under stress conditions. The local or systemic control of BNF and the role played by reactive oxygen species (ROS) in such regulation have still not been elucidated completely. Cadmium, which belongs to the so-called heavy metals, is one of the most toxic substances released into the environment. The mechanisms involved in Cd toxicity are still not completely understood but the overproduction of ROS is one of its characteristic symptoms. In this work, we used a split-root system approach to study nodule BNF and the antioxidant machinery’s response to the application of a mild Cd treatment on one side of a nodulated Medicago truncatula root system. Cd induced the majority of nodule antioxidants without generating any oxidative damage. Cd treatment also provoked BNF inhibition exclusively in nodules directly exposed to Cd, without provoking any effect on plant shoot biomass or chlorophyll content. The overall data suggest that the decline in BNF was not due to a generalized breakdown of the plant but to control exerted through leghaemoglobin/oxygen availability, affecting nitrogenase function.
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spelling pubmed-38718182013-12-26 Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin Marino, Daniel Damiani, Isabelle Gucciardo, Sébastien Mijangos, Iker Pauly, Nicolas Puppo, Alain J Exp Bot Research Paper Leguminous biological nitrogen fixation (BNF) is very sensitive to environmental fluctuations. It is still contentious how BNF is regulated under stress conditions. The local or systemic control of BNF and the role played by reactive oxygen species (ROS) in such regulation have still not been elucidated completely. Cadmium, which belongs to the so-called heavy metals, is one of the most toxic substances released into the environment. The mechanisms involved in Cd toxicity are still not completely understood but the overproduction of ROS is one of its characteristic symptoms. In this work, we used a split-root system approach to study nodule BNF and the antioxidant machinery’s response to the application of a mild Cd treatment on one side of a nodulated Medicago truncatula root system. Cd induced the majority of nodule antioxidants without generating any oxidative damage. Cd treatment also provoked BNF inhibition exclusively in nodules directly exposed to Cd, without provoking any effect on plant shoot biomass or chlorophyll content. The overall data suggest that the decline in BNF was not due to a generalized breakdown of the plant but to control exerted through leghaemoglobin/oxygen availability, affecting nitrogenase function. Oxford University Press 2013-12 2013-10-22 /pmc/articles/PMC3871818/ /pubmed/24151304 http://dx.doi.org/10.1093/jxb/ert334 Text en © The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Marino, Daniel
Damiani, Isabelle
Gucciardo, Sébastien
Mijangos, Iker
Pauly, Nicolas
Puppo, Alain
Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title_full Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title_fullStr Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title_full_unstemmed Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title_short Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin
title_sort inhibition of nitrogen fixation in symbiotic medicago truncatula upon cd exposure is a local process involving leghaemoglobin
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871818/
https://www.ncbi.nlm.nih.gov/pubmed/24151304
http://dx.doi.org/10.1093/jxb/ert334
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