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Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4

Endothelial dysfunction is a critical factor during the initiation of cardiovascular complications in diabetes. Berberine can ameliorate endothelial dysfunction induced by diabetes. However, the underlying mechanisms remain unclear. The aim of this study was to investigate the protective effect and...

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Autores principales: Zhang, Ming, Wang, Chun-Mei, Li, Jing, Meng, Zhao-Jie, Wei, Sheng-Nan, Li, Ji, Bucala, Richard, Li, Yu-Lin, Chen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872165/
https://www.ncbi.nlm.nih.gov/pubmed/24385682
http://dx.doi.org/10.1155/2013/260464
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author Zhang, Ming
Wang, Chun-Mei
Li, Jing
Meng, Zhao-Jie
Wei, Sheng-Nan
Li, Ji
Bucala, Richard
Li, Yu-Lin
Chen, Li
author_facet Zhang, Ming
Wang, Chun-Mei
Li, Jing
Meng, Zhao-Jie
Wei, Sheng-Nan
Li, Ji
Bucala, Richard
Li, Yu-Lin
Chen, Li
author_sort Zhang, Ming
collection PubMed
description Endothelial dysfunction is a critical factor during the initiation of cardiovascular complications in diabetes. Berberine can ameliorate endothelial dysfunction induced by diabetes. However, the underlying mechanisms remain unclear. The aim of this study was to investigate the protective effect and mechanism of berberine on palmitate-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs). The cell viability of HUVECs was determined by MTT assays. Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs. The mRNA level of endothelial nitric oxide synthase (eNOS) was measured by RT-PCR, and the protein levels of eNOS, p-eNOS, Akt, p-Akt, AMPK, p-AMPK, and NADPH oxidase (NOX4) were analyzed. The results demonstrated that berberine significantly elevated NO levels and reduced the production of ROS. The expressions of eNOS were significantly increased, while NOX4 protein expression was decreased in berberine-treated HUVECs. Moreover, berberine upregulated the protein expression of AMPK and p-AMPK in palmitate-treated HUVECs, but had no effect on the levels of Akt. Therefore, berberine ameliorates palmitate-induced endothelial dysfunction by upregulating eNOS expression and downregulating expression of NOX4. This regulatory effect of berberine may be related to the activation of AMPK.
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spelling pubmed-38721652014-01-02 Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4 Zhang, Ming Wang, Chun-Mei Li, Jing Meng, Zhao-Jie Wei, Sheng-Nan Li, Ji Bucala, Richard Li, Yu-Lin Chen, Li Mediators Inflamm Research Article Endothelial dysfunction is a critical factor during the initiation of cardiovascular complications in diabetes. Berberine can ameliorate endothelial dysfunction induced by diabetes. However, the underlying mechanisms remain unclear. The aim of this study was to investigate the protective effect and mechanism of berberine on palmitate-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs). The cell viability of HUVECs was determined by MTT assays. Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs. The mRNA level of endothelial nitric oxide synthase (eNOS) was measured by RT-PCR, and the protein levels of eNOS, p-eNOS, Akt, p-Akt, AMPK, p-AMPK, and NADPH oxidase (NOX4) were analyzed. The results demonstrated that berberine significantly elevated NO levels and reduced the production of ROS. The expressions of eNOS were significantly increased, while NOX4 protein expression was decreased in berberine-treated HUVECs. Moreover, berberine upregulated the protein expression of AMPK and p-AMPK in palmitate-treated HUVECs, but had no effect on the levels of Akt. Therefore, berberine ameliorates palmitate-induced endothelial dysfunction by upregulating eNOS expression and downregulating expression of NOX4. This regulatory effect of berberine may be related to the activation of AMPK. Hindawi Publishing Corporation 2013 2013-12-09 /pmc/articles/PMC3872165/ /pubmed/24385682 http://dx.doi.org/10.1155/2013/260464 Text en Copyright © 2013 Ming Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Ming
Wang, Chun-Mei
Li, Jing
Meng, Zhao-Jie
Wei, Sheng-Nan
Li, Ji
Bucala, Richard
Li, Yu-Lin
Chen, Li
Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title_full Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title_fullStr Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title_full_unstemmed Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title_short Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4
title_sort berberine protects against palmitate-induced endothelial dysfunction: involvements of upregulation of ampk and enos and downregulation of nox4
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872165/
https://www.ncbi.nlm.nih.gov/pubmed/24385682
http://dx.doi.org/10.1155/2013/260464
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