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Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation

Alveolar epithelial type II cells (AECIIs) containing lamellar bodies (LBs) are alveolar epithelial stem cells that have important functions in the repair of lung structure and function after lung injury. The ultrastructural changes in AECIIs after high-frequency oscillatory ventilation (HFOV) with...

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Autores principales: Qin, Xiaofei, Fu, Wanhai, Zhao, Youwei, Meng, Qiong, You, Chuming, Yu, Qiuming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872375/
https://www.ncbi.nlm.nih.gov/pubmed/24386636
http://dx.doi.org/10.1155/2013/240659
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author Qin, Xiaofei
Fu, Wanhai
Zhao, Youwei
Meng, Qiong
You, Chuming
Yu, Qiuming
author_facet Qin, Xiaofei
Fu, Wanhai
Zhao, Youwei
Meng, Qiong
You, Chuming
Yu, Qiuming
author_sort Qin, Xiaofei
collection PubMed
description Alveolar epithelial type II cells (AECIIs) containing lamellar bodies (LBs) are alveolar epithelial stem cells that have important functions in the repair of lung structure and function after lung injury. The ultrastructural changes in AECIIs after high-frequency oscillatory ventilation (HFOV) with a high lung volume strategy or conventional ventilation were evaluated in a newborn piglet model with acute lung injury (ALI). After ALI with saline lavage, newborn piglets were randomly assigned into five study groups (three piglets in each group), namely, control (no mechanical ventilation), conventional ventilation for 24 h, conventional ventilation for 48 h, HFOV for 24 h, and HFOV for 48 h. The lower tissues of the right lung were obtained to observe the AECII ultrastructure. AECIIs with reduced numbers of microvilli, decreased LBs electron density, and vacuole-like LBs deformity were commonly observed in all five groups. Compared with conventional ventilation groups, the decrease in numbers of microvilli and LBs electron density, as well as LBs with vacuole-like appearance and polymorphic deformity, was less severe in HFOV with high lung volume strategy groups. AECIIs were injured during mechanical ventilation. HFOV with a high lung volume strategy resulted in less AECII damage than conventional ventilation.
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spelling pubmed-38723752014-01-02 Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation Qin, Xiaofei Fu, Wanhai Zhao, Youwei Meng, Qiong You, Chuming Yu, Qiuming Biomed Res Int Research Article Alveolar epithelial type II cells (AECIIs) containing lamellar bodies (LBs) are alveolar epithelial stem cells that have important functions in the repair of lung structure and function after lung injury. The ultrastructural changes in AECIIs after high-frequency oscillatory ventilation (HFOV) with a high lung volume strategy or conventional ventilation were evaluated in a newborn piglet model with acute lung injury (ALI). After ALI with saline lavage, newborn piglets were randomly assigned into five study groups (three piglets in each group), namely, control (no mechanical ventilation), conventional ventilation for 24 h, conventional ventilation for 48 h, HFOV for 24 h, and HFOV for 48 h. The lower tissues of the right lung were obtained to observe the AECII ultrastructure. AECIIs with reduced numbers of microvilli, decreased LBs electron density, and vacuole-like LBs deformity were commonly observed in all five groups. Compared with conventional ventilation groups, the decrease in numbers of microvilli and LBs electron density, as well as LBs with vacuole-like appearance and polymorphic deformity, was less severe in HFOV with high lung volume strategy groups. AECIIs were injured during mechanical ventilation. HFOV with a high lung volume strategy resulted in less AECII damage than conventional ventilation. Hindawi Publishing Corporation 2013 2013-12-10 /pmc/articles/PMC3872375/ /pubmed/24386636 http://dx.doi.org/10.1155/2013/240659 Text en Copyright © 2013 Xiaofei Qin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qin, Xiaofei
Fu, Wanhai
Zhao, Youwei
Meng, Qiong
You, Chuming
Yu, Qiuming
Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title_full Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title_fullStr Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title_full_unstemmed Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title_short Ultrastructural Study of Alveolar Epithelial Type II Cells by High-Frequency Oscillatory Ventilation
title_sort ultrastructural study of alveolar epithelial type ii cells by high-frequency oscillatory ventilation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872375/
https://www.ncbi.nlm.nih.gov/pubmed/24386636
http://dx.doi.org/10.1155/2013/240659
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