Genetic and epigenetic effects of salinity on in vitro growth of barley

Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreas...

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Autores principales: Demirkiran, Aykut, Marakli, Sevgi, Temel, Aslihan, Gozukirmizi, Nermin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2013
Materias:
Plant Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873189/
https://www.ncbi.nlm.nih.gov/pubmed/24385861
http://dx.doi.org/10.1590/S1415-47572013000400016
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author Demirkiran, Aykut
Marakli, Sevgi
Temel, Aslihan
Gozukirmizi, Nermin
author_facet Demirkiran, Aykut
Marakli, Sevgi
Temel, Aslihan
Gozukirmizi, Nermin
author_sort Demirkiran, Aykut
collection PubMed
description Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
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spelling pubmed-38731892014-01-02 Genetic and epigenetic effects of salinity on in vitro growth of barley Demirkiran, Aykut Marakli, Sevgi Temel, Aslihan Gozukirmizi, Nermin Genet Mol Biol Plant Genetics Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley. Sociedade Brasileira de Genética 2013-12 2013-11-08 /pmc/articles/PMC3873189/ /pubmed/24385861 http://dx.doi.org/10.1590/S1415-47572013000400016 Text en Copyright © 2013, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics
Demirkiran, Aykut
Marakli, Sevgi
Temel, Aslihan
Gozukirmizi, Nermin
Genetic and epigenetic effects of salinity on in vitro growth of barley
title Genetic and epigenetic effects of salinity on in vitro growth of barley
title_full Genetic and epigenetic effects of salinity on in vitro growth of barley
title_fullStr Genetic and epigenetic effects of salinity on in vitro growth of barley
title_full_unstemmed Genetic and epigenetic effects of salinity on in vitro growth of barley
title_short Genetic and epigenetic effects of salinity on in vitro growth of barley
title_sort genetic and epigenetic effects of salinity on in vitro growth of barley
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873189/
https://www.ncbi.nlm.nih.gov/pubmed/24385861
http://dx.doi.org/10.1590/S1415-47572013000400016
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