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ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity

The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-...

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Autores principales: Spallek, Thomas, Beck, Martina, Ben Khaled, Sara, Salomon, Susanne, Bourdais, Gildas, Schellmann, Swen, Robatzek, Silke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873229/
https://www.ncbi.nlm.nih.gov/pubmed/24385929
http://dx.doi.org/10.1371/journal.pgen.1004035
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author Spallek, Thomas
Beck, Martina
Ben Khaled, Sara
Salomon, Susanne
Bourdais, Gildas
Schellmann, Swen
Robatzek, Silke
author_facet Spallek, Thomas
Beck, Martina
Ben Khaled, Sara
Salomon, Susanne
Bourdais, Gildas
Schellmann, Swen
Robatzek, Silke
author_sort Spallek, Thomas
collection PubMed
description The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity.
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spelling pubmed-38732292014-01-02 ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity Spallek, Thomas Beck, Martina Ben Khaled, Sara Salomon, Susanne Bourdais, Gildas Schellmann, Swen Robatzek, Silke PLoS Genet Research Article The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and is internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis in Arabidopsis. VPS37-1 co-localizes with FLS2 at endosomes and immunoprecipitates with the receptor upon flg22 elicitation. Vps37-1 mutants are reduced in flg22-induced FLS2 endosomes but not in endosomes labeled by Rab5 GTPases suggesting a defect in FLS2 trafficking rather than formation of endosomes. FLS2 localizes to the lumen of multivesicular bodies, but this is altered in vps37-1 mutants indicating compromised endosomal sorting of FLS2 by ESCRT-I loss-of-function. VPS37-1 and VPS28-2 are critical for immunity against bacterial infection through a role in stomatal closure. Our findings identify that VPS37-1, and likewise VPS28-2, regulate late FLS2 endosomal sorting and reveals that ESCRT-I is critical for flg22-activated stomatal defenses involved in plant immunity. Public Library of Science 2013-12-26 /pmc/articles/PMC3873229/ /pubmed/24385929 http://dx.doi.org/10.1371/journal.pgen.1004035 Text en © 2013 Spallek et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Spallek, Thomas
Beck, Martina
Ben Khaled, Sara
Salomon, Susanne
Bourdais, Gildas
Schellmann, Swen
Robatzek, Silke
ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title_full ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title_fullStr ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title_full_unstemmed ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title_short ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
title_sort escrt-i mediates fls2 endosomal sorting and plant immunity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873229/
https://www.ncbi.nlm.nih.gov/pubmed/24385929
http://dx.doi.org/10.1371/journal.pgen.1004035
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