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Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions
During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, rema...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873251/ https://www.ncbi.nlm.nih.gov/pubmed/24385939 http://dx.doi.org/10.1371/journal.pgen.1004071 |
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author | Copsey, Alice Tang, Shangming Jordan, Philip W. Blitzblau, Hannah G. Newcombe, Sonya Chan, Andrew Chi-ho Newnham, Louise Li, Zhaobo Gray, Stephen Herbert, Alex D. Arumugam, Prakash Hochwagen, Andreas Hunter, Neil Hoffmann, Eva |
author_facet | Copsey, Alice Tang, Shangming Jordan, Philip W. Blitzblau, Hannah G. Newcombe, Sonya Chan, Andrew Chi-ho Newnham, Louise Li, Zhaobo Gray, Stephen Herbert, Alex D. Arumugam, Prakash Hochwagen, Andreas Hunter, Neil Hoffmann, Eva |
author_sort | Copsey, Alice |
collection | PubMed |
description | During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutLγ complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4(Eme1). Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe. |
format | Online Article Text |
id | pubmed-3873251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38732512014-01-02 Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions Copsey, Alice Tang, Shangming Jordan, Philip W. Blitzblau, Hannah G. Newcombe, Sonya Chan, Andrew Chi-ho Newnham, Louise Li, Zhaobo Gray, Stephen Herbert, Alex D. Arumugam, Prakash Hochwagen, Andreas Hunter, Neil Hoffmann, Eva PLoS Genet Research Article During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutLγ complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4(Eme1). Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe. Public Library of Science 2013-12-26 /pmc/articles/PMC3873251/ /pubmed/24385939 http://dx.doi.org/10.1371/journal.pgen.1004071 Text en © 2013 Copsey et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Copsey, Alice Tang, Shangming Jordan, Philip W. Blitzblau, Hannah G. Newcombe, Sonya Chan, Andrew Chi-ho Newnham, Louise Li, Zhaobo Gray, Stephen Herbert, Alex D. Arumugam, Prakash Hochwagen, Andreas Hunter, Neil Hoffmann, Eva Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title | Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title_full | Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title_fullStr | Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title_full_unstemmed | Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title_short | Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions |
title_sort | smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873251/ https://www.ncbi.nlm.nih.gov/pubmed/24385939 http://dx.doi.org/10.1371/journal.pgen.1004071 |
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