Determination of HER2 Amplification Status on Tumour DNA by Digital PCR

Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromoso...

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Detalles Bibliográficos
Autores principales: Garcia-Murillas, Isaac, Lambros, Maryou, Turner, Nicholas C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873285/
https://www.ncbi.nlm.nih.gov/pubmed/24386193
http://dx.doi.org/10.1371/journal.pone.0083409
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author Garcia-Murillas, Isaac
Lambros, Maryou
Turner, Nicholas C.
author_facet Garcia-Murillas, Isaac
Lambros, Maryou
Turner, Nicholas C.
author_sort Garcia-Murillas, Isaac
collection PubMed
description Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2:EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.
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spelling pubmed-38732852014-01-02 Determination of HER2 Amplification Status on Tumour DNA by Digital PCR Garcia-Murillas, Isaac Lambros, Maryou Turner, Nicholas C. PLoS One Research Article Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2:EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status. Public Library of Science 2013-12-26 /pmc/articles/PMC3873285/ /pubmed/24386193 http://dx.doi.org/10.1371/journal.pone.0083409 Text en © 2013 Garcia-Murillas et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Garcia-Murillas, Isaac
Lambros, Maryou
Turner, Nicholas C.
Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title_full Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title_fullStr Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title_full_unstemmed Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title_short Determination of HER2 Amplification Status on Tumour DNA by Digital PCR
title_sort determination of her2 amplification status on tumour dna by digital pcr
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873285/
https://www.ncbi.nlm.nih.gov/pubmed/24386193
http://dx.doi.org/10.1371/journal.pone.0083409
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