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ISEV position paper: extracellular vesicle RNA analysis and bioinformatics

Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in...

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Autores principales: Hill, Andrew F., Pegtel, D. Michiel, Lambertz, Ulrike, Leonardi, Tommaso, O'Driscoll, Lorraine, Pluchino, Stefano, Ter-Ovanesyan, Dmitry, Nolte-‘t Hoen, Esther N.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873759/
https://www.ncbi.nlm.nih.gov/pubmed/24376909
http://dx.doi.org/10.3402/jev.v2i0.22859
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author Hill, Andrew F.
Pegtel, D. Michiel
Lambertz, Ulrike
Leonardi, Tommaso
O'Driscoll, Lorraine
Pluchino, Stefano
Ter-Ovanesyan, Dmitry
Nolte-‘t Hoen, Esther N.M.
author_facet Hill, Andrew F.
Pegtel, D. Michiel
Lambertz, Ulrike
Leonardi, Tommaso
O'Driscoll, Lorraine
Pluchino, Stefano
Ter-Ovanesyan, Dmitry
Nolte-‘t Hoen, Esther N.M.
author_sort Hill, Andrew F.
collection PubMed
description Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on “evRNA analysis and bioinformatics.” Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis.
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spelling pubmed-38737592013-12-27 ISEV position paper: extracellular vesicle RNA analysis and bioinformatics Hill, Andrew F. Pegtel, D. Michiel Lambertz, Ulrike Leonardi, Tommaso O'Driscoll, Lorraine Pluchino, Stefano Ter-Ovanesyan, Dmitry Nolte-‘t Hoen, Esther N.M. J Extracell Vesicles Position Paper Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on “evRNA analysis and bioinformatics.” Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis. Co-Action Publishing 2013-12-23 /pmc/articles/PMC3873759/ /pubmed/24376909 http://dx.doi.org/10.3402/jev.v2i0.22859 Text en © 2013 Andrew F. Hill et al. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Position Paper
Hill, Andrew F.
Pegtel, D. Michiel
Lambertz, Ulrike
Leonardi, Tommaso
O'Driscoll, Lorraine
Pluchino, Stefano
Ter-Ovanesyan, Dmitry
Nolte-‘t Hoen, Esther N.M.
ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title_full ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title_fullStr ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title_full_unstemmed ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title_short ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
title_sort isev position paper: extracellular vesicle rna analysis and bioinformatics
topic Position Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873759/
https://www.ncbi.nlm.nih.gov/pubmed/24376909
http://dx.doi.org/10.3402/jev.v2i0.22859
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