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ISEV position paper: extracellular vesicle RNA analysis and bioinformatics
Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873759/ https://www.ncbi.nlm.nih.gov/pubmed/24376909 http://dx.doi.org/10.3402/jev.v2i0.22859 |
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author | Hill, Andrew F. Pegtel, D. Michiel Lambertz, Ulrike Leonardi, Tommaso O'Driscoll, Lorraine Pluchino, Stefano Ter-Ovanesyan, Dmitry Nolte-‘t Hoen, Esther N.M. |
author_facet | Hill, Andrew F. Pegtel, D. Michiel Lambertz, Ulrike Leonardi, Tommaso O'Driscoll, Lorraine Pluchino, Stefano Ter-Ovanesyan, Dmitry Nolte-‘t Hoen, Esther N.M. |
author_sort | Hill, Andrew F. |
collection | PubMed |
description | Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on “evRNA analysis and bioinformatics.” Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis. |
format | Online Article Text |
id | pubmed-3873759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-38737592013-12-27 ISEV position paper: extracellular vesicle RNA analysis and bioinformatics Hill, Andrew F. Pegtel, D. Michiel Lambertz, Ulrike Leonardi, Tommaso O'Driscoll, Lorraine Pluchino, Stefano Ter-Ovanesyan, Dmitry Nolte-‘t Hoen, Esther N.M. J Extracell Vesicles Position Paper Extracellular vesicles (EVs) are the collective term for the various vesicles that are released by cells into the extracellular space. Such vesicles include exosomes and microvesicles, which vary by their size and/or protein and genetic cargo. With the discovery that EVs contain genetic material in the form of RNA (evRNA) has come the increased interest in these vesicles for their potential use as sources of disease biomarkers and potential therapeutic agents. Rapid developments in the availability of deep sequencing technologies have enabled the study of EV-related RNA in detail. In October 2012, the International Society for Extracellular Vesicles (ISEV) held a workshop on “evRNA analysis and bioinformatics.” Here, we report the conclusions of one of the roundtable discussions where we discussed evRNA analysis technologies and provide some guidelines to researchers in the field to consider when performing such analysis. Co-Action Publishing 2013-12-23 /pmc/articles/PMC3873759/ /pubmed/24376909 http://dx.doi.org/10.3402/jev.v2i0.22859 Text en © 2013 Andrew F. Hill et al. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Position Paper Hill, Andrew F. Pegtel, D. Michiel Lambertz, Ulrike Leonardi, Tommaso O'Driscoll, Lorraine Pluchino, Stefano Ter-Ovanesyan, Dmitry Nolte-‘t Hoen, Esther N.M. ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title | ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title_full | ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title_fullStr | ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title_full_unstemmed | ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title_short | ISEV position paper: extracellular vesicle RNA analysis and bioinformatics |
title_sort | isev position paper: extracellular vesicle rna analysis and bioinformatics |
topic | Position Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873759/ https://www.ncbi.nlm.nih.gov/pubmed/24376909 http://dx.doi.org/10.3402/jev.v2i0.22859 |
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