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Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization

Homotypic membrane tethering by the Golgi reassembly and stacking proteins (GRASPs) is required for the lateral linkage of mammalian Golgi ministacks into a ribbon-like membrane network. Although GRASP65 and GRASP55 are specifically localized to cis and medial/trans cisternae, respectively, it is un...

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Autores principales: Jarvela, Timothy, Linstedt, Adam D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873884/
https://www.ncbi.nlm.nih.gov/pubmed/24227884
http://dx.doi.org/10.1091/mbc.E13-07-0395
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author Jarvela, Timothy
Linstedt, Adam D.
author_facet Jarvela, Timothy
Linstedt, Adam D.
author_sort Jarvela, Timothy
collection PubMed
description Homotypic membrane tethering by the Golgi reassembly and stacking proteins (GRASPs) is required for the lateral linkage of mammalian Golgi ministacks into a ribbon-like membrane network. Although GRASP65 and GRASP55 are specifically localized to cis and medial/trans cisternae, respectively, it is unknown whether each GRASP mediates cisternae-specific tethering and whether such specificity is necessary for Golgi compartmentalization. Here each GRASP was tagged with KillerRed (KR), expressed in HeLa cells, and inhibited by 1-min exposure to light. Significantly, inactivation of either GRASP unlinked the Golgi ribbon, and the immediate effect of GRASP65-KR inactivation was a loss of cis- rather than trans-Golgi integrity, whereas inactivation of GRASP55-KR first affected the trans- and not the cis-Golgi. Thus each GRASP appears to play a direct and cisternae-specific role in linking ministacks into a continuous membrane network. To test the consequence of loss of cisternae-specific tethering, we generated Golgi membranes with a single GRASP on all cisternae. Remarkably, the membranes exhibited the full connectivity of wild-type Golgi ribbons but were decompartmentalized and defective in glycan processing. Thus the GRASP isoforms specifically link analogous cisternae to ensure Golgi compartmentalization and proper processing.
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spelling pubmed-38738842014-03-16 Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization Jarvela, Timothy Linstedt, Adam D. Mol Biol Cell Articles Homotypic membrane tethering by the Golgi reassembly and stacking proteins (GRASPs) is required for the lateral linkage of mammalian Golgi ministacks into a ribbon-like membrane network. Although GRASP65 and GRASP55 are specifically localized to cis and medial/trans cisternae, respectively, it is unknown whether each GRASP mediates cisternae-specific tethering and whether such specificity is necessary for Golgi compartmentalization. Here each GRASP was tagged with KillerRed (KR), expressed in HeLa cells, and inhibited by 1-min exposure to light. Significantly, inactivation of either GRASP unlinked the Golgi ribbon, and the immediate effect of GRASP65-KR inactivation was a loss of cis- rather than trans-Golgi integrity, whereas inactivation of GRASP55-KR first affected the trans- and not the cis-Golgi. Thus each GRASP appears to play a direct and cisternae-specific role in linking ministacks into a continuous membrane network. To test the consequence of loss of cisternae-specific tethering, we generated Golgi membranes with a single GRASP on all cisternae. Remarkably, the membranes exhibited the full connectivity of wild-type Golgi ribbons but were decompartmentalized and defective in glycan processing. Thus the GRASP isoforms specifically link analogous cisternae to ensure Golgi compartmentalization and proper processing. The American Society for Cell Biology 2014-01-01 /pmc/articles/PMC3873884/ /pubmed/24227884 http://dx.doi.org/10.1091/mbc.E13-07-0395 Text en © 2014 Jarvela and Linstedt. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Jarvela, Timothy
Linstedt, Adam D.
Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title_full Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title_fullStr Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title_full_unstemmed Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title_short Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization
title_sort isoform-specific tethering links the golgi ribbon to maintain compartmentalization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873884/
https://www.ncbi.nlm.nih.gov/pubmed/24227884
http://dx.doi.org/10.1091/mbc.E13-07-0395
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