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Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides

Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipi...

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Autores principales: Nishimura, Shinichi, Ishii, Kumiko, Iwamoto, Kunihiko, Arita, Yuko, Matsunaga, Shigeki, Ohno-Iwashita, Yoshiko, Sato, Satoshi B., Kakeya, Hideaki, Kobayashi, Toshihide, Yoshida, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873978/
https://www.ncbi.nlm.nih.gov/pubmed/24386262
http://dx.doi.org/10.1371/journal.pone.0083716
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author Nishimura, Shinichi
Ishii, Kumiko
Iwamoto, Kunihiko
Arita, Yuko
Matsunaga, Shigeki
Ohno-Iwashita, Yoshiko
Sato, Satoshi B.
Kakeya, Hideaki
Kobayashi, Toshihide
Yoshida, Minoru
author_facet Nishimura, Shinichi
Ishii, Kumiko
Iwamoto, Kunihiko
Arita, Yuko
Matsunaga, Shigeki
Ohno-Iwashita, Yoshiko
Sato, Satoshi B.
Kakeya, Hideaki
Kobayashi, Toshihide
Yoshida, Minoru
author_sort Nishimura, Shinichi
collection PubMed
description Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions.
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spelling pubmed-38739782014-01-02 Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides Nishimura, Shinichi Ishii, Kumiko Iwamoto, Kunihiko Arita, Yuko Matsunaga, Shigeki Ohno-Iwashita, Yoshiko Sato, Satoshi B. Kakeya, Hideaki Kobayashi, Toshihide Yoshida, Minoru PLoS One Research Article Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions. Public Library of Science 2013-12-27 /pmc/articles/PMC3873978/ /pubmed/24386262 http://dx.doi.org/10.1371/journal.pone.0083716 Text en © 2013 Nishimura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nishimura, Shinichi
Ishii, Kumiko
Iwamoto, Kunihiko
Arita, Yuko
Matsunaga, Shigeki
Ohno-Iwashita, Yoshiko
Sato, Satoshi B.
Kakeya, Hideaki
Kobayashi, Toshihide
Yoshida, Minoru
Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title_full Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title_fullStr Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title_full_unstemmed Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title_short Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
title_sort visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873978/
https://www.ncbi.nlm.nih.gov/pubmed/24386262
http://dx.doi.org/10.1371/journal.pone.0083716
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