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Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873978/ https://www.ncbi.nlm.nih.gov/pubmed/24386262 http://dx.doi.org/10.1371/journal.pone.0083716 |
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author | Nishimura, Shinichi Ishii, Kumiko Iwamoto, Kunihiko Arita, Yuko Matsunaga, Shigeki Ohno-Iwashita, Yoshiko Sato, Satoshi B. Kakeya, Hideaki Kobayashi, Toshihide Yoshida, Minoru |
author_facet | Nishimura, Shinichi Ishii, Kumiko Iwamoto, Kunihiko Arita, Yuko Matsunaga, Shigeki Ohno-Iwashita, Yoshiko Sato, Satoshi B. Kakeya, Hideaki Kobayashi, Toshihide Yoshida, Minoru |
author_sort | Nishimura, Shinichi |
collection | PubMed |
description | Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions. |
format | Online Article Text |
id | pubmed-3873978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38739782014-01-02 Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides Nishimura, Shinichi Ishii, Kumiko Iwamoto, Kunihiko Arita, Yuko Matsunaga, Shigeki Ohno-Iwashita, Yoshiko Sato, Satoshi B. Kakeya, Hideaki Kobayashi, Toshihide Yoshida, Minoru PLoS One Research Article Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions. Public Library of Science 2013-12-27 /pmc/articles/PMC3873978/ /pubmed/24386262 http://dx.doi.org/10.1371/journal.pone.0083716 Text en © 2013 Nishimura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Nishimura, Shinichi Ishii, Kumiko Iwamoto, Kunihiko Arita, Yuko Matsunaga, Shigeki Ohno-Iwashita, Yoshiko Sato, Satoshi B. Kakeya, Hideaki Kobayashi, Toshihide Yoshida, Minoru Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title | Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title_full | Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title_fullStr | Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title_full_unstemmed | Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title_short | Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides |
title_sort | visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873978/ https://www.ncbi.nlm.nih.gov/pubmed/24386262 http://dx.doi.org/10.1371/journal.pone.0083716 |
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