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Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells
Objective(s): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells. Materials and Methods: Seven kinds of bacteria were reconstituted, inoculated and tested in t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874095/ https://www.ncbi.nlm.nih.gov/pubmed/24379966 |
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author | Huang, Zehao Ren, Jianwu |
author_facet | Huang, Zehao Ren, Jianwu |
author_sort | Huang, Zehao |
collection | PubMed |
description | Objective(s): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells. Materials and Methods: Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U2OS human osteosarcoma cells was unveiled. Rerults: The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (12.5%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G0/G1 ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U2OS, through preventing the transition from G1 phase to S phase. It reduced osteosarcoma cell, U2OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation. Conclusion: Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells. |
format | Online Article Text |
id | pubmed-3874095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-38740952013-12-30 Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells Huang, Zehao Ren, Jianwu Iran J Basic Med Sci Original Article Objective(s): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells. Materials and Methods: Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U2OS human osteosarcoma cells was unveiled. Rerults: The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (12.5%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G0/G1 ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U2OS, through preventing the transition from G1 phase to S phase. It reduced osteosarcoma cell, U2OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation. Conclusion: Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells. Mashhad University of Medical Sciences 2013-10 /pmc/articles/PMC3874095/ /pubmed/24379966 Text en © 2013: Iranian Journal of Basic Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Huang, Zehao Ren, Jianwu Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title | Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title_full | Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title_fullStr | Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title_full_unstemmed | Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title_short | Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells |
title_sort | antibacterial activity of elephant garlic and its effect against u2os human osteosarcoma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874095/ https://www.ncbi.nlm.nih.gov/pubmed/24379966 |
work_keys_str_mv | AT huangzehao antibacterialactivityofelephantgarlicanditseffectagainstu2oshumanosteosarcomacells AT renjianwu antibacterialactivityofelephantgarlicanditseffectagainstu2oshumanosteosarcomacells |