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Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia

Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) is the commonest leukemia in adults. Here, we aimed to evaluate hsa-miR-15a/hsa-miR-16-1 expression in CLL tissues by qPCR and correlate it with the other clinicopathological features and clinical outcome. 40 formalin-fixed paraffin-embed...

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Autores principales: Rossi, Maura, Fuligni, Fabio, Ciccone, Maria, Agostinelli, Claudio, Righi, Simona, Luciani, Marco, Laginestra, Maria Antonella, Rigolin, Gian Matteo, Sapienza, Maria Rosaria, Gazzola, Anna, Mannu, Claudia, Cuneo, Antonio, Pileri, Stefano, Piccaluga, Pier Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874364/
https://www.ncbi.nlm.nih.gov/pubmed/24392455
http://dx.doi.org/10.1155/2013/715391
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author Rossi, Maura
Fuligni, Fabio
Ciccone, Maria
Agostinelli, Claudio
Righi, Simona
Luciani, Marco
Laginestra, Maria Antonella
Rigolin, Gian Matteo
Sapienza, Maria Rosaria
Gazzola, Anna
Mannu, Claudia
Cuneo, Antonio
Pileri, Stefano
Piccaluga, Pier Paolo
author_facet Rossi, Maura
Fuligni, Fabio
Ciccone, Maria
Agostinelli, Claudio
Righi, Simona
Luciani, Marco
Laginestra, Maria Antonella
Rigolin, Gian Matteo
Sapienza, Maria Rosaria
Gazzola, Anna
Mannu, Claudia
Cuneo, Antonio
Pileri, Stefano
Piccaluga, Pier Paolo
author_sort Rossi, Maura
collection PubMed
description Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) is the commonest leukemia in adults. Here, we aimed to evaluate hsa-miR-15a/hsa-miR-16-1 expression in CLL tissues by qPCR and correlate it with the other clinicopathological features and clinical outcome. 40 formalin-fixed paraffin-embedded (FFPE) lymph node samples obtained from CLL/SLL patients were classified into two categories, “PCs rich” and “typical.” We found a significant common expression level of 4 miRNAs; however, we did not find any significant relationship between PCs presence and miRNAs expression. Moreover, neither the presence of 13q deletion nor the percentage of cells carrying the deletion strictly correlated with miRNAs expression levels, although a significant number of patients with 13q deletion presented hsa-miR-16-1-3p levels below the median value in normal samples (P < 0.05). Finally, although no correlation was found between the expression of each miRNA and other clinicopathological features (Ki67, CD38, ZAP70, and IGVH@ hypermutations), the OS curves showed a positive trend in patients with miRNAs downregulation, though not statistically significant. In conclusion, we showed for the first time that all miRNAs can be successfully studied in FFPE CLL tissues and that del13q and PCs richness do not strictly correspond to miRNAs downregulation; therefore, a specific evaluation may be envisaged at least in patients enrolled in clinical trials.
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spelling pubmed-38743642014-01-05 Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia Rossi, Maura Fuligni, Fabio Ciccone, Maria Agostinelli, Claudio Righi, Simona Luciani, Marco Laginestra, Maria Antonella Rigolin, Gian Matteo Sapienza, Maria Rosaria Gazzola, Anna Mannu, Claudia Cuneo, Antonio Pileri, Stefano Piccaluga, Pier Paolo Biomed Res Int Research Article Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) is the commonest leukemia in adults. Here, we aimed to evaluate hsa-miR-15a/hsa-miR-16-1 expression in CLL tissues by qPCR and correlate it with the other clinicopathological features and clinical outcome. 40 formalin-fixed paraffin-embedded (FFPE) lymph node samples obtained from CLL/SLL patients were classified into two categories, “PCs rich” and “typical.” We found a significant common expression level of 4 miRNAs; however, we did not find any significant relationship between PCs presence and miRNAs expression. Moreover, neither the presence of 13q deletion nor the percentage of cells carrying the deletion strictly correlated with miRNAs expression levels, although a significant number of patients with 13q deletion presented hsa-miR-16-1-3p levels below the median value in normal samples (P < 0.05). Finally, although no correlation was found between the expression of each miRNA and other clinicopathological features (Ki67, CD38, ZAP70, and IGVH@ hypermutations), the OS curves showed a positive trend in patients with miRNAs downregulation, though not statistically significant. In conclusion, we showed for the first time that all miRNAs can be successfully studied in FFPE CLL tissues and that del13q and PCs richness do not strictly correspond to miRNAs downregulation; therefore, a specific evaluation may be envisaged at least in patients enrolled in clinical trials. Hindawi Publishing Corporation 2013 2013-12-12 /pmc/articles/PMC3874364/ /pubmed/24392455 http://dx.doi.org/10.1155/2013/715391 Text en Copyright © 2013 Maura Rossi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rossi, Maura
Fuligni, Fabio
Ciccone, Maria
Agostinelli, Claudio
Righi, Simona
Luciani, Marco
Laginestra, Maria Antonella
Rigolin, Gian Matteo
Sapienza, Maria Rosaria
Gazzola, Anna
Mannu, Claudia
Cuneo, Antonio
Pileri, Stefano
Piccaluga, Pier Paolo
Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title_full Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title_fullStr Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title_full_unstemmed Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title_short Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
title_sort hsa-mir-15a and hsa-mir-16-1 expression is not related to proliferation centers abundance and other prognostic factors in chronic lymphocytic leukemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874364/
https://www.ncbi.nlm.nih.gov/pubmed/24392455
http://dx.doi.org/10.1155/2013/715391
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