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In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis

Zingiber officinale is a traditional medicine against various disorders including liver diseases.The aim of this study was to assess the hepatoprotective activity of the ethanolic extract of rhizomes of Z. officinale (ERZO) against thioacetamide-induced hepatotoxicity in rats. Five groups of male Sp...

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Autores principales: Abdulaziz Bardi, Daleya, Halabi, Mohammed Farouq, Abdullah, Nor Azizan, Rouhollahi, Elham, Hajrezaie, Maryam, Abdulla, Mahmood Ameen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874366/
https://www.ncbi.nlm.nih.gov/pubmed/24396831
http://dx.doi.org/10.1155/2013/918460
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author Abdulaziz Bardi, Daleya
Halabi, Mohammed Farouq
Abdullah, Nor Azizan
Rouhollahi, Elham
Hajrezaie, Maryam
Abdulla, Mahmood Ameen
author_facet Abdulaziz Bardi, Daleya
Halabi, Mohammed Farouq
Abdullah, Nor Azizan
Rouhollahi, Elham
Hajrezaie, Maryam
Abdulla, Mahmood Ameen
author_sort Abdulaziz Bardi, Daleya
collection PubMed
description Zingiber officinale is a traditional medicine against various disorders including liver diseases.The aim of this study was to assess the hepatoprotective activity of the ethanolic extract of rhizomes of Z. officinale (ERZO) against thioacetamide-induced hepatotoxicity in rats. Five groups of male Sprague Dawley have been used. In group 1 rats received intraperitoneal (i.p.) injection of normal saline while groups 2–5 received thioacetamide (TAA, 200 mg/kg; i.p.) for induction of liver cirrhosis, thrice weekly for eight weeks. Group 3 received 50 mg/kg of silymarin. The rats in groups 4 and 5 received 250 and 500 mg/kg of ERZO (dissolved in 10% Tween), respectively. Hepatic damage was assessed grossly and microscopically for all of the groups. Results confirmed the induction of liver cirrhosis in group 2 whilst administration of silymarin or ERZO significantly reduced the impact of thioacetamide toxicity. These groups decreased fibrosis of the liver tissues. Immunohistochemistry assessment against proliferating cell nuclear antigen did not show remarkable proliferation in the ERZO-treated rats when compared with group 2. Moreover, factions of the ERZO extract were tested on Hep-G2 cells and showed antiproliferative activity (IC(50) 38–60 μg/mL). This study showed hepatoprotective effect of ERZO.
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spelling pubmed-38743662014-01-06 In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis Abdulaziz Bardi, Daleya Halabi, Mohammed Farouq Abdullah, Nor Azizan Rouhollahi, Elham Hajrezaie, Maryam Abdulla, Mahmood Ameen Biomed Res Int Research Article Zingiber officinale is a traditional medicine against various disorders including liver diseases.The aim of this study was to assess the hepatoprotective activity of the ethanolic extract of rhizomes of Z. officinale (ERZO) against thioacetamide-induced hepatotoxicity in rats. Five groups of male Sprague Dawley have been used. In group 1 rats received intraperitoneal (i.p.) injection of normal saline while groups 2–5 received thioacetamide (TAA, 200 mg/kg; i.p.) for induction of liver cirrhosis, thrice weekly for eight weeks. Group 3 received 50 mg/kg of silymarin. The rats in groups 4 and 5 received 250 and 500 mg/kg of ERZO (dissolved in 10% Tween), respectively. Hepatic damage was assessed grossly and microscopically for all of the groups. Results confirmed the induction of liver cirrhosis in group 2 whilst administration of silymarin or ERZO significantly reduced the impact of thioacetamide toxicity. These groups decreased fibrosis of the liver tissues. Immunohistochemistry assessment against proliferating cell nuclear antigen did not show remarkable proliferation in the ERZO-treated rats when compared with group 2. Moreover, factions of the ERZO extract were tested on Hep-G2 cells and showed antiproliferative activity (IC(50) 38–60 μg/mL). This study showed hepatoprotective effect of ERZO. Hindawi Publishing Corporation 2013 2013-12-12 /pmc/articles/PMC3874366/ /pubmed/24396831 http://dx.doi.org/10.1155/2013/918460 Text en Copyright © 2013 Daleya Abdulaziz Bardi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abdulaziz Bardi, Daleya
Halabi, Mohammed Farouq
Abdullah, Nor Azizan
Rouhollahi, Elham
Hajrezaie, Maryam
Abdulla, Mahmood Ameen
In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title_full In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title_fullStr In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title_full_unstemmed In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title_short In Vivo Evaluation of Ethanolic Extract of Zingiber officinale Rhizomes for Its Protective Effect against Liver Cirrhosis
title_sort in vivo evaluation of ethanolic extract of zingiber officinale rhizomes for its protective effect against liver cirrhosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874366/
https://www.ncbi.nlm.nih.gov/pubmed/24396831
http://dx.doi.org/10.1155/2013/918460
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