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In vitro irradiation system for radiobiological experiments

BACKGROUND: Although two-dimensional (2-D) monolayer cell cultures provide important information on basic tumor biology and radiobiology, they are not representative of the complexity of three-dimensional (3-D) solid tumors. In particular, new models reproducing clinical conditions as closely as pos...

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Autores principales: Tesei, Anna, Sarnelli, Anna, Arienti, Chiara, Menghi, Enrico, Medri, Laura, Gabucci, Elisa, Pignatta, Sara, Falconi, Mirella, Silvestrini, Rosella, Zoli, Wainer, D’Errico, Vincenzo, Romeo, Antonino, Parisi, Elisabetta, Polico, Rolando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874638/
https://www.ncbi.nlm.nih.gov/pubmed/24180359
http://dx.doi.org/10.1186/1748-717X-8-257
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author Tesei, Anna
Sarnelli, Anna
Arienti, Chiara
Menghi, Enrico
Medri, Laura
Gabucci, Elisa
Pignatta, Sara
Falconi, Mirella
Silvestrini, Rosella
Zoli, Wainer
D’Errico, Vincenzo
Romeo, Antonino
Parisi, Elisabetta
Polico, Rolando
author_facet Tesei, Anna
Sarnelli, Anna
Arienti, Chiara
Menghi, Enrico
Medri, Laura
Gabucci, Elisa
Pignatta, Sara
Falconi, Mirella
Silvestrini, Rosella
Zoli, Wainer
D’Errico, Vincenzo
Romeo, Antonino
Parisi, Elisabetta
Polico, Rolando
author_sort Tesei, Anna
collection PubMed
description BACKGROUND: Although two-dimensional (2-D) monolayer cell cultures provide important information on basic tumor biology and radiobiology, they are not representative of the complexity of three-dimensional (3-D) solid tumors. In particular, new models reproducing clinical conditions as closely as possible are needed for radiobiological studies to provide information that can be translated from bench to bedside. METHODS: We developed a novel system for the irradiation, under sterile conditions, of 3-D tumor spheroids, the in vitro model considered as a bridge between the complex architectural organization of in vivo tumors and the very simple one of in vitro monolayer cell cultures. The system exploits the same equipment as that used for patient treatments, without the need for dedicated and highly expensive instruments. To mimic the passage of radiation beams through human tissues before they reach the target tumor mass, 96-multiwell plates containing the multicellular tumor spheroids (MCTS) are inserted into a custom-built phantom made of plexiglass, the material most similar to water, the main component of human tissue. RESULTS: The system was used to irradiate CAEP- and A549-derived MCTS, pre-treated or not with 20 μM cisplatin, with a dose of 20 Gy delivered in one session. We also tested the same treatment schemes on monolayer CAEP and A549 cells. Our preliminary results indicated a significant increment in radiotoxicity 20 days after the end of irradiation in the CAEP spheroids pre-treated with cisplatin compared to those treated with cisplatin or irradiation alone. Conversely, the effect of the radio- chemotherapy combination in A549-derived MCTS was similar to that induced by cisplatin or irradiation alone. Finally, the 20 Gy dose did not affect cell survival in monolayer CAEP and A549 cells, whereas cisplatin or cisplatin plus radiation caused 100% cell death, regardless of the type of cell line used. CONCLUSIONS: We set up a system for the irradiation, under sterile conditions, of tumor cells grown in 3-D which allows for the use of the same dose intensities and schedules utilized in clinical practice. This irradiation system, coupled with 3-D cell cultures, has the potential to generate information that could be used to individually tailor radiotherapy.
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spelling pubmed-38746382013-12-31 In vitro irradiation system for radiobiological experiments Tesei, Anna Sarnelli, Anna Arienti, Chiara Menghi, Enrico Medri, Laura Gabucci, Elisa Pignatta, Sara Falconi, Mirella Silvestrini, Rosella Zoli, Wainer D’Errico, Vincenzo Romeo, Antonino Parisi, Elisabetta Polico, Rolando Radiat Oncol Research BACKGROUND: Although two-dimensional (2-D) monolayer cell cultures provide important information on basic tumor biology and radiobiology, they are not representative of the complexity of three-dimensional (3-D) solid tumors. In particular, new models reproducing clinical conditions as closely as possible are needed for radiobiological studies to provide information that can be translated from bench to bedside. METHODS: We developed a novel system for the irradiation, under sterile conditions, of 3-D tumor spheroids, the in vitro model considered as a bridge between the complex architectural organization of in vivo tumors and the very simple one of in vitro monolayer cell cultures. The system exploits the same equipment as that used for patient treatments, without the need for dedicated and highly expensive instruments. To mimic the passage of radiation beams through human tissues before they reach the target tumor mass, 96-multiwell plates containing the multicellular tumor spheroids (MCTS) are inserted into a custom-built phantom made of plexiglass, the material most similar to water, the main component of human tissue. RESULTS: The system was used to irradiate CAEP- and A549-derived MCTS, pre-treated or not with 20 μM cisplatin, with a dose of 20 Gy delivered in one session. We also tested the same treatment schemes on monolayer CAEP and A549 cells. Our preliminary results indicated a significant increment in radiotoxicity 20 days after the end of irradiation in the CAEP spheroids pre-treated with cisplatin compared to those treated with cisplatin or irradiation alone. Conversely, the effect of the radio- chemotherapy combination in A549-derived MCTS was similar to that induced by cisplatin or irradiation alone. Finally, the 20 Gy dose did not affect cell survival in monolayer CAEP and A549 cells, whereas cisplatin or cisplatin plus radiation caused 100% cell death, regardless of the type of cell line used. CONCLUSIONS: We set up a system for the irradiation, under sterile conditions, of tumor cells grown in 3-D which allows for the use of the same dose intensities and schedules utilized in clinical practice. This irradiation system, coupled with 3-D cell cultures, has the potential to generate information that could be used to individually tailor radiotherapy. BioMed Central 2013-11-01 /pmc/articles/PMC3874638/ /pubmed/24180359 http://dx.doi.org/10.1186/1748-717X-8-257 Text en Copyright © 2013 Tesei et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Tesei, Anna
Sarnelli, Anna
Arienti, Chiara
Menghi, Enrico
Medri, Laura
Gabucci, Elisa
Pignatta, Sara
Falconi, Mirella
Silvestrini, Rosella
Zoli, Wainer
D’Errico, Vincenzo
Romeo, Antonino
Parisi, Elisabetta
Polico, Rolando
In vitro irradiation system for radiobiological experiments
title In vitro irradiation system for radiobiological experiments
title_full In vitro irradiation system for radiobiological experiments
title_fullStr In vitro irradiation system for radiobiological experiments
title_full_unstemmed In vitro irradiation system for radiobiological experiments
title_short In vitro irradiation system for radiobiological experiments
title_sort in vitro irradiation system for radiobiological experiments
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874638/
https://www.ncbi.nlm.nih.gov/pubmed/24180359
http://dx.doi.org/10.1186/1748-717X-8-257
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