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Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens

BACKGROUND: Adipogenesis from preadipocytes into mature adipocyte is precisely coordinated by transcription factors such as CCAAT-enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ), cytokines, and hormones, which is accompanied by extracellular matrix remodel...

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Autores principales: Kim, Byoungjae, Choi, Kyung Min, Yim, Hong Soon, Lee, Min-Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874642/
https://www.ncbi.nlm.nih.gov/pubmed/24325571
http://dx.doi.org/10.1186/1476-511X-12-182
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author Kim, Byoungjae
Choi, Kyung Min
Yim, Hong Soon
Lee, Min-Goo
author_facet Kim, Byoungjae
Choi, Kyung Min
Yim, Hong Soon
Lee, Min-Goo
author_sort Kim, Byoungjae
collection PubMed
description BACKGROUND: Adipogenesis from preadipocytes into mature adipocyte is precisely coordinated by transcription factors such as CCAAT-enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ), cytokines, and hormones, which is accompanied by extracellular matrix remodeling. Besides anti-oxidant activity, ascorbic acid (ASC) is participating in collagen biosynthesis and increase production and processing of collagens. Moreover, several studies demonstrated that ASC enhanced differentiation from preadipocytes into mature adipocytes. METHODS: The adipogenic effect of ascorbic acid was evaluated in chemical induced 3T3-L1 by Oil Red O staining. This effect was elucidated by immunoblotting which detected the expression level of collagens and transcription factors in adipogenesis. The immunocytochemical determination of type I collagen was performed in 3T3-L1 adipocyte to show the change of extracellular matrix during adipogenesis. RESULTS: In this study, Oil Red O staining in 3T3-L1 preadipocytes was increased dose-dependently by addition of ASC. These ASC-treated adipocytes increased collagen processing of α1(I) and α1(V) and expressed α1(VI) and α2(VI) collagens differentially. ASC also stimulated expression of C/EBPα and PPARγ, which is preceded by collagen enhancement. In addition, inhibition of ASC activity by ethyl-3,4-dihydroxybenzoate showed reduction of lipid accumulation by removal of large lipid droplets, not by inhibition of lipid production. This observation went with loss of α1(I) deposition on adipocyte surface, increase of α1(V) and α2(VI) collagens and decrease of C/EBPs. CONCLUSION: Our findings imply that various actions of ASC on adipogenesis through differential collagen expression may provide diverse applications of ASC to adipose tissue technology.
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spelling pubmed-38746422013-12-31 Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens Kim, Byoungjae Choi, Kyung Min Yim, Hong Soon Lee, Min-Goo Lipids Health Dis Research BACKGROUND: Adipogenesis from preadipocytes into mature adipocyte is precisely coordinated by transcription factors such as CCAAT-enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ), cytokines, and hormones, which is accompanied by extracellular matrix remodeling. Besides anti-oxidant activity, ascorbic acid (ASC) is participating in collagen biosynthesis and increase production and processing of collagens. Moreover, several studies demonstrated that ASC enhanced differentiation from preadipocytes into mature adipocytes. METHODS: The adipogenic effect of ascorbic acid was evaluated in chemical induced 3T3-L1 by Oil Red O staining. This effect was elucidated by immunoblotting which detected the expression level of collagens and transcription factors in adipogenesis. The immunocytochemical determination of type I collagen was performed in 3T3-L1 adipocyte to show the change of extracellular matrix during adipogenesis. RESULTS: In this study, Oil Red O staining in 3T3-L1 preadipocytes was increased dose-dependently by addition of ASC. These ASC-treated adipocytes increased collagen processing of α1(I) and α1(V) and expressed α1(VI) and α2(VI) collagens differentially. ASC also stimulated expression of C/EBPα and PPARγ, which is preceded by collagen enhancement. In addition, inhibition of ASC activity by ethyl-3,4-dihydroxybenzoate showed reduction of lipid accumulation by removal of large lipid droplets, not by inhibition of lipid production. This observation went with loss of α1(I) deposition on adipocyte surface, increase of α1(V) and α2(VI) collagens and decrease of C/EBPs. CONCLUSION: Our findings imply that various actions of ASC on adipogenesis through differential collagen expression may provide diverse applications of ASC to adipose tissue technology. BioMed Central 2013-12-11 /pmc/articles/PMC3874642/ /pubmed/24325571 http://dx.doi.org/10.1186/1476-511X-12-182 Text en Copyright © 2013 Kim et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kim, Byoungjae
Choi, Kyung Min
Yim, Hong Soon
Lee, Min-Goo
Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title_full Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title_fullStr Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title_full_unstemmed Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title_short Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagens
title_sort ascorbic acid enhances adipogenesis of 3t3-l1 murine preadipocyte through differential expression of collagens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874642/
https://www.ncbi.nlm.nih.gov/pubmed/24325571
http://dx.doi.org/10.1186/1476-511X-12-182
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