Cargando…
Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis
BACKGROUND: Renal artery stenosis (RAS) promotes hypertension and cardiac dysfunction. The 2-kidney, 1-clip mouse model in many ways resembles RAS in humans and is amenable for genetic manipulation, but difficult to evaluate noninvasively. We hypothesized that cardiovascular magnetic resonance (CMR)...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874758/ https://www.ncbi.nlm.nih.gov/pubmed/24160179 http://dx.doi.org/10.1186/1532-429X-15-98 |
_version_ | 1782297271775264768 |
---|---|
author | Ebrahimi, Behzad Crane, John A Knudsen, Bruce E Macura, Slobodan I Grande, Joseph P Lerman, Lilach O |
author_facet | Ebrahimi, Behzad Crane, John A Knudsen, Bruce E Macura, Slobodan I Grande, Joseph P Lerman, Lilach O |
author_sort | Ebrahimi, Behzad |
collection | PubMed |
description | BACKGROUND: Renal artery stenosis (RAS) promotes hypertension and cardiac dysfunction. The 2-kidney, 1-clip mouse model in many ways resembles RAS in humans and is amenable for genetic manipulation, but difficult to evaluate noninvasively. We hypothesized that cardiovascular magnetic resonance (CMR) is capable of detecting progressive cardiac and renal dysfunction in mice with RAS and monitoring the progression of the disease longitudinally. METHODS: RAS was induced at baseline in eighteen mice by constricting the renal artery. Nine additional animals served as normal controls. CMR scans (16.4 T) were performed in all mice one week before and 2 and 4 weeks after baseline. Renal volumes and hemodynamics were assessed using 3D fast imaging with steady-state precession and arterial spin labelling, and cardiac function using CMR cine. Renal hypoxia was investigated using blood oxygen-level dependent (BOLD) MR. RESULTS: Two weeks after surgery, mean arterial pressure was elevated in RAS mice. The stenotic kidney (STK) showed atrophy, while the contra-lateral kidney (CLK) showed hypertrophy. Renal blood flow (RBF) and cortical oxygenation level declined in the STK but remained unchanged in CLK. Moreover, cardiac end-diastolic and stroke volumes decreased and myocardial mass increased. At 4 weeks, STK RBF remained declined and the STK cortex and medulla showed development of hypoxia. Additionally, BOLD detected a mild hypoxia in CLK cortex. Cardiac end-diastolic and stroke volumes remained reduced and left ventricular hypertrophy worsened. Left ventricular filling velocities (E/A) indicated progression of cardiac dysfunction towards restrictive filling. CONCLUSIONS: CMR detected longitudinal progression of cardiac and renal dysfunction in 2K, 1C mice. These observations support the use of high-field CMR to obtain useful information regarding chronic cardiac and renal dysfunction in small animals. |
format | Online Article Text |
id | pubmed-3874758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38747582013-12-31 Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis Ebrahimi, Behzad Crane, John A Knudsen, Bruce E Macura, Slobodan I Grande, Joseph P Lerman, Lilach O J Cardiovasc Magn Reson Research BACKGROUND: Renal artery stenosis (RAS) promotes hypertension and cardiac dysfunction. The 2-kidney, 1-clip mouse model in many ways resembles RAS in humans and is amenable for genetic manipulation, but difficult to evaluate noninvasively. We hypothesized that cardiovascular magnetic resonance (CMR) is capable of detecting progressive cardiac and renal dysfunction in mice with RAS and monitoring the progression of the disease longitudinally. METHODS: RAS was induced at baseline in eighteen mice by constricting the renal artery. Nine additional animals served as normal controls. CMR scans (16.4 T) were performed in all mice one week before and 2 and 4 weeks after baseline. Renal volumes and hemodynamics were assessed using 3D fast imaging with steady-state precession and arterial spin labelling, and cardiac function using CMR cine. Renal hypoxia was investigated using blood oxygen-level dependent (BOLD) MR. RESULTS: Two weeks after surgery, mean arterial pressure was elevated in RAS mice. The stenotic kidney (STK) showed atrophy, while the contra-lateral kidney (CLK) showed hypertrophy. Renal blood flow (RBF) and cortical oxygenation level declined in the STK but remained unchanged in CLK. Moreover, cardiac end-diastolic and stroke volumes decreased and myocardial mass increased. At 4 weeks, STK RBF remained declined and the STK cortex and medulla showed development of hypoxia. Additionally, BOLD detected a mild hypoxia in CLK cortex. Cardiac end-diastolic and stroke volumes remained reduced and left ventricular hypertrophy worsened. Left ventricular filling velocities (E/A) indicated progression of cardiac dysfunction towards restrictive filling. CONCLUSIONS: CMR detected longitudinal progression of cardiac and renal dysfunction in 2K, 1C mice. These observations support the use of high-field CMR to obtain useful information regarding chronic cardiac and renal dysfunction in small animals. BioMed Central 2013-10-26 /pmc/articles/PMC3874758/ /pubmed/24160179 http://dx.doi.org/10.1186/1532-429X-15-98 Text en Copyright © 2013 Ebrahimi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ebrahimi, Behzad Crane, John A Knudsen, Bruce E Macura, Slobodan I Grande, Joseph P Lerman, Lilach O Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title | Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title_full | Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title_fullStr | Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title_full_unstemmed | Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title_short | Evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
title_sort | evolution of cardiac and renal impairment detected by high-field cardiovascular magnetic resonance in mice with renal artery stenosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874758/ https://www.ncbi.nlm.nih.gov/pubmed/24160179 http://dx.doi.org/10.1186/1532-429X-15-98 |
work_keys_str_mv | AT ebrahimibehzad evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis AT cranejohna evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis AT knudsenbrucee evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis AT macuraslobodani evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis AT grandejosephp evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis AT lermanlilacho evolutionofcardiacandrenalimpairmentdetectedbyhighfieldcardiovascularmagneticresonanceinmicewithrenalarterystenosis |