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Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution

Synthetic seeds were formed from shoot tips of two in vitro grown Begonia cultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant...

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Autores principales: Sakhanokho, Hamidou F., Pounders, Cecil T., Blythe, Eugene K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875128/
https://www.ncbi.nlm.nih.gov/pubmed/24396296
http://dx.doi.org/10.1155/2013/341568
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author Sakhanokho, Hamidou F.
Pounders, Cecil T.
Blythe, Eugene K.
author_facet Sakhanokho, Hamidou F.
Pounders, Cecil T.
Blythe, Eugene K.
author_sort Sakhanokho, Hamidou F.
collection PubMed
description Synthetic seeds were formed from shoot tips of two in vitro grown Begonia cultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant combination into 100 mM calcium chloride (CaCl(2) ·H(2)O) solution for 30 or 45 min. Both control and encapsulated shoots were transferred into sterile Petri dishes and stored at 4°C or 22°C for 0, 2, 4, 6, or 8 weeks. Conversion of synthetic seeds into plantlets for both storage environments was assessed in MS medium or peat-based substrate. No significant difference was found between the 30 and 45 min CaCl(2) ·H(2)O treatments or the two cultivars. Encapsulation of explants improved survival rate over time irrespective of the medium type or storage environment. Survival rates of 88, 53, 28, and 11% for encapsulated microshoots versus 73, 13, 0, and 0% for control explants were achieved in microshoots stored for 2, 4, 6, and 8 weeks, respectively. The best results were obtained when synthetic seeds were stored at 4°C and germinated on MS medium. Regenerated plantlets were successfully established in potting soil.
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spelling pubmed-38751282014-01-06 Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution Sakhanokho, Hamidou F. Pounders, Cecil T. Blythe, Eugene K. ScientificWorldJournal Research Article Synthetic seeds were formed from shoot tips of two in vitro grown Begonia cultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant combination into 100 mM calcium chloride (CaCl(2) ·H(2)O) solution for 30 or 45 min. Both control and encapsulated shoots were transferred into sterile Petri dishes and stored at 4°C or 22°C for 0, 2, 4, 6, or 8 weeks. Conversion of synthetic seeds into plantlets for both storage environments was assessed in MS medium or peat-based substrate. No significant difference was found between the 30 and 45 min CaCl(2) ·H(2)O treatments or the two cultivars. Encapsulation of explants improved survival rate over time irrespective of the medium type or storage environment. Survival rates of 88, 53, 28, and 11% for encapsulated microshoots versus 73, 13, 0, and 0% for control explants were achieved in microshoots stored for 2, 4, 6, and 8 weeks, respectively. The best results were obtained when synthetic seeds were stored at 4°C and germinated on MS medium. Regenerated plantlets were successfully established in potting soil. Hindawi Publishing Corporation 2013-12-12 /pmc/articles/PMC3875128/ /pubmed/24396296 http://dx.doi.org/10.1155/2013/341568 Text en Copyright © 2013 Hamidou F. Sakhanokho et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sakhanokho, Hamidou F.
Pounders, Cecil T.
Blythe, Eugene K.
Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title_full Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title_fullStr Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title_full_unstemmed Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title_short Alginate Encapsulation of Begonia Microshoots for Short-Term Storage and Distribution
title_sort alginate encapsulation of begonia microshoots for short-term storage and distribution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875128/
https://www.ncbi.nlm.nih.gov/pubmed/24396296
http://dx.doi.org/10.1155/2013/341568
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