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Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography
Efficient carbon utilization is critical to the survival of microorganisms in competitive environments. To optimize energy usage, bacteria have developed an integrated control system to preferentially uptake carbohydrates that support rapid growth. The availability of a preferred carbon source such...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875231/ https://www.ncbi.nlm.nih.gov/pubmed/23770568 http://dx.doi.org/10.1038/nature12232 |
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author | Chen, Shanshuang Oldham, Michael L. Davidson, Amy L. Chen, Jue |
author_facet | Chen, Shanshuang Oldham, Michael L. Davidson, Amy L. Chen, Jue |
author_sort | Chen, Shanshuang |
collection | PubMed |
description | Efficient carbon utilization is critical to the survival of microorganisms in competitive environments. To optimize energy usage, bacteria have developed an integrated control system to preferentially uptake carbohydrates that support rapid growth. The availability of a preferred carbon source such as glucose represses the synthesis and activities of proteins necessary for the transport and metabolism of secondary carbon sources. This regulatory phenomenon is defined as carbon catabolite repression (CCR)(1). In enteric bacteria, the key player of CCR is a component of the glucose-specific phosphotransferase system, enzyme IIA (EIIA(Glc))(1,2). It is known that unphosphorylated EIIA(Glc) binds and inhibits a variety of transporters when glucose is available(1,2). However, understanding the underlying molecular mechanism has been hindered by the complete absence of structures for any EIIA(Glc)-transporter complexes. Here, we present the 3.9 Å crystal structure of EIIA(Glc) in complex with the maltose transporter, an ATP-binding cassette (ABC) transporter. The structure shows that two EIIA(Glc) molecules bind to the cytoplasmic ATPase subunits, stabilizing the transporter in an inward-facing conformation and preventing the structural rearrangements necessary for ATP hydrolysis. We also show that the half maximal inhibitory concentrations of the full-length EIIA(Glc) and an N-terminal truncation mutant differ by 60 fold, consistent with the hypothesis that the N-terminal region, disordered in the crystal structure, functions as a membrane-anchor to increase the effective EIIA(Glc) concentration at the membrane(3,4). Together these data suggest a model of how the central regulatory protein EIIA(Glc) allosterically inhibits maltose uptake in E. coli. |
format | Online Article Text |
id | pubmed-3875231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-38752312014-01-18 Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography Chen, Shanshuang Oldham, Michael L. Davidson, Amy L. Chen, Jue Nature Article Efficient carbon utilization is critical to the survival of microorganisms in competitive environments. To optimize energy usage, bacteria have developed an integrated control system to preferentially uptake carbohydrates that support rapid growth. The availability of a preferred carbon source such as glucose represses the synthesis and activities of proteins necessary for the transport and metabolism of secondary carbon sources. This regulatory phenomenon is defined as carbon catabolite repression (CCR)(1). In enteric bacteria, the key player of CCR is a component of the glucose-specific phosphotransferase system, enzyme IIA (EIIA(Glc))(1,2). It is known that unphosphorylated EIIA(Glc) binds and inhibits a variety of transporters when glucose is available(1,2). However, understanding the underlying molecular mechanism has been hindered by the complete absence of structures for any EIIA(Glc)-transporter complexes. Here, we present the 3.9 Å crystal structure of EIIA(Glc) in complex with the maltose transporter, an ATP-binding cassette (ABC) transporter. The structure shows that two EIIA(Glc) molecules bind to the cytoplasmic ATPase subunits, stabilizing the transporter in an inward-facing conformation and preventing the structural rearrangements necessary for ATP hydrolysis. We also show that the half maximal inhibitory concentrations of the full-length EIIA(Glc) and an N-terminal truncation mutant differ by 60 fold, consistent with the hypothesis that the N-terminal region, disordered in the crystal structure, functions as a membrane-anchor to increase the effective EIIA(Glc) concentration at the membrane(3,4). Together these data suggest a model of how the central regulatory protein EIIA(Glc) allosterically inhibits maltose uptake in E. coli. 2013-06-16 2013-07-18 /pmc/articles/PMC3875231/ /pubmed/23770568 http://dx.doi.org/10.1038/nature12232 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Chen, Shanshuang Oldham, Michael L. Davidson, Amy L. Chen, Jue Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title | Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title_full | Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title_fullStr | Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title_full_unstemmed | Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title_short | Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography |
title_sort | carbon catabolite repression of the maltose transporter revealed by x-ray crystallography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875231/ https://www.ncbi.nlm.nih.gov/pubmed/23770568 http://dx.doi.org/10.1038/nature12232 |
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